Guo Hang, Ekusa Ai, Iwai Koji, Yonekura Masami, Takahata Yoshihisa, Morimatsu Fumiki
R & D Center, Nippon Meat Packers, Inc., Tsukuba, Ibaraki, Japan.
J Nutr Sci Vitaminol (Tokyo). 2008 Jun;54(3):191-5. doi: 10.3177/jnsv.54.191.
Royal jelly peptides (RJPx) isolated from hydrolysates of water-soluble royal jelly proteins prepared with protease P exhibited significantly stronger hydroxyl radical-scavenging activity (p<0.001), and antioxidant activity against lipid peroxidation (LPO, p<0.001), than did water-soluble royal jelly protein (WSRJP) in vitro. We also investigated the in vivo antioxidant activity of RJPx against ferric nitrilotriacetate (Fe-NTA)-induced LPO. Male Wistar rats were divided into a control group (Group C), an Fe-NTA group (Group Fe), and an Fe-NTA with RJPx group (Group Fe+R). Rats in Group Fe+R were fed RJPx (2 g/kg body weight) daily for 5 wk. Fe-NTA (8 mg Fe/kg body weight) was then intraperitoneally injected, and serum lipid levels were examined 2 h later. Serum total cholesterol (TC) levels were lower (p<0.05) while low-density lipoprotein (LDL) and LPO were significantly higher (p<0.01) in Group Fe than in Group C. TC (p<0.05) and LPO levels (p<0.01) were lower in Group Fe+R than in Group Fe. Our data suggest that RJPx may inhibit LPO both in vitro and in vivo.
从用蛋白酶P制备的水溶性蜂王浆蛋白水解物中分离出的蜂王浆肽（RJPx）在体外表现出比水溶性蜂王浆蛋白（WSRJP）更强的羟自由基清除活性（p<0.001）和抗脂质过氧化（LPO，p<0.001）的抗氧化活性。我们还研究了RJPx对次氮基三乙酸铁（Fe-NTA）诱导的LPO的体内抗氧化活性。将雄性Wistar大鼠分为对照组（C组）、Fe-NTA组（Fe组）和Fe-NTA加RJPx组（Fe+R组）。Fe+R组大鼠每天喂食RJPx（2 g/kg体重），持续5周。然后腹腔注射Fe-NTA（8 mg Fe/kg体重），2小时后检测血清脂质水平。Fe组血清总胆固醇（TC）水平低于C组（p<0.05），而低密度脂蛋白（LDL）和LPO显著高于C组（p<0.01）。Fe+R组的TC（p<0.05）和LPO水平（p<0.01）低于Fe组。我们的数据表明，RJPx可能在体外和体内均抑制LPO。
