Arai Makoto, Imazeki Fumio, Sakai Yuzo, Mikata Rintaro, Tada Motohisa, Seki Naohiko, Shimada Hideaki, Ochiai Takenori, Yokosuka Osamu
Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Oncol Rep. 2008 Aug;20(2):405-12.
To determine the clinical significance of gene promoter methylation in esophageal squamous cell carcinoma (ESCC), we examined the promoter methylation status of genes showing elevated expression, as determined by DNA microarray-based transcriptomic analysis, in three ESCC cell lines (TE-1, TE-2, TE-10) after 5-aza-2'-deoxycytidine (DAC) treatment. We observed a high degree of DNA methylation within the promoter regions of three genes, namely cathepsin L2 (CTSL2), normal mucosa of esophagus specific 1 (NMES1), and fatty acid binding protein 5 (FABP5). Overexpression of NMES1 in ESCC cell lines increased cell motility. Down-regulation of NMES1 might play an important role in the cell motility of ESCC or be a potent marker of malignancy.
为了确定基因启动子甲基化在食管鳞状细胞癌（ESCC）中的临床意义，我们检测了经5-氮杂-2'-脱氧胞苷（DAC）处理后的三种ESCC细胞系（TE-1、TE-2、TE-10）中，基于DNA微阵列的转录组分析显示表达升高的基因的启动子甲基化状态。我们观察到组织蛋白酶L2（CTSL2）、食管正常黏膜特异性1（NMES1）和脂肪酸结合蛋白5（FABP5）这三个基因的启动子区域存在高度DNA甲基化。ESCC细胞系中NMES1的过表达增加了细胞运动性。NMES1的下调可能在ESCC的细胞运动中起重要作用，或者是恶性肿瘤的一个有效标志物。
