Li Li-Yi, Li Ji-Kun, Shen Yun, Yu Liang, Zhang Jian-Hai
Department of General Surgery, The Second Affiliated Hospital, Wenzhou Medical College, Wenzhou, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2008 Jul;11(4):379-82.
To analyze the relationships among the aberrant methylation of Runx3 gene promoter, the Runx3 protein expression and clinicopathological features in gastric cancer.
Methylation specific PCR was used to measure the promoter methylation status of Runx3 gene in tumor and the adjacent normal mucosal tissues from 40 patients with gastric cancer. Protein expression of Runx3 was measured by immunohistochemistry.
The frequency of promoter methylation of Runx3 gene in gastric cancer tissue(55.0%) was significantly higher compared to the adjacent normal tissues (12.5%)(P<0.01). The positive rate of protein expression of Runx3 in gastric cancer tissue(37.5%) was significantly lower compared to the adjacent normal tissues (100%). There was marked association between hypermethylation and negative protein expression (P<0.05). The frequency of Runx3 promoter methylation was associated with histological type, N grade, and tumor stage.
The promoter hypermethylation is a main mechanism of reduced or loss expression of Runx3 gene, which may provide molecular diagnosis and stage evaluation of gastric cancer.
分析胃癌中Runx3基因启动子异常甲基化、Runx3蛋白表达与临床病理特征之间的关系。
采用甲基化特异性PCR检测40例胃癌患者肿瘤组织及癌旁正常黏膜组织中Runx3基因启动子的甲基化状态。采用免疫组织化学法检测Runx3蛋白表达。
胃癌组织中Runx3基因启动子甲基化频率（55.0%）显著高于癌旁正常组织（12.5%）（P<0.01）。胃癌组织中Runx3蛋白表达阳性率（37.5%）显著低于癌旁正常组织（100%）。高甲基化与蛋白表达阴性之间存在显著关联（P<0.05）。Runx3启动子甲基化频率与组织学类型、N分级和肿瘤分期有关。
启动子高甲基化是Runx3基因表达降低或缺失的主要机制，可为胃癌的分子诊断和分期评估提供依据。
