Dainichiseika Color & Chemicals Mfg. Co., Ltd., 1-9-4, Horinouchi, Adachi-ku, Tokyo 123, Japan.
Biotechnol Bioeng. 1997 Oct 20;56(2):162-7. doi: 10.1002/(SICI)1097-0290(19971020)56:2<162::AID-BIT5>3.0.CO;2-N.
The integration of a charged membrane into a perstraction system for high selective separation is reported. A mixture of N-(benzyloxycarbonyl)-L-aspartic acid (ZA), L-phenylalanine methyl ester (PM), and N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester (ZAPM) was used as the model solution. The aqueous phase containing ZA, PM, and ZAPM was adjusted to pH 6 and was contacted with tert-amyl alcohol through a charged membrane. Seven different ion-exchange membranes and two different microfiltration membranes were tested for the separation system. Only ZAPM could permeate into the organic phase through SELEMION AMV and ASV. The separations between ZA and ZAPM and between PM and ZAPM were performed by biphasic extraction and electrostatic rejection, respectively. The permeabilities of ZAPM were higher than those of PM for all experiments using the ion-exchange membranes, although the molecular weight of ZAPM is larger than that of PM. The membrane that had a smaller pore size showed higher ZAPM selectivity. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 162-167, 1997.
报告了将荷电膜集成到用于高选择性分离的超滤体系中。以 N-（苄氧羰基）-L-天冬氨酸（ZA）、L-苯丙氨酸甲酯（PM）和 N-（苄氧羰基）-L-天冬氨酰基-L-苯丙氨酸甲酯（ZAPM）的混合物作为模型溶液。将含有 ZA、PM 和 ZAPM 的水相调节至 pH 6，并通过荷电膜与叔戊醇接触。测试了七种不同的离子交换膜和两种不同的微滤膜用于分离体系。只有 ZAPM 可以通过 SELEMION AMV 和 ASV 渗透到有机相中。ZA 和 ZAPM 之间以及 PM 和 ZAPM 之间的分离分别通过两相萃取和静电排斥进行。对于使用离子交换膜进行的所有实验，ZAPM 的渗透率均高于 PM，尽管 ZAPM 的分子量大于 PM。孔径较小的膜显示出更高的 ZAPM 选择性。（c）1997 年 John Wiley & Sons，Inc. 《生物技术与生物工程》56：162-167，1997。
