Lippi Giuseppe, Schena Federico, Montagnana Martina, Salvagno Gian Luca, Guidi Gian Cesare
Sezione di Chimica Clinica, Dipartimento di Scienze Morfologico-Biomediche, Università di Verona, Verona, Italy.
Clin Chem Lab Med. 2008;46(9):1313-8. doi: 10.1515/CCLM.2008.250.
Although there is comprehensive information on traditional biomarkers of muscle and cardiac damage following exercise, less is known on the kinetics of innovative markers, including ischemia modified albumin (IMA), glycogen phosphorylase isoenzyme BB (GPBB), carbonic anhydrase III (CAIII) and heart-type fatty acid-binding protein (H-FABP) in athletes performing a sub-maximal exercise.
A total of 10 healthy trained Caucasian males performed a 21-km run. Blood samples were collected before the run, immediately after (post), 3, 6 and 24 h thereafter. Cardiac troponin I (cTnI), myoglobin, creatine kinase isoenzyme MB (CK-MB), GPBB, CAIII and H-FABP were assayed using a new diagnostic system based on protein biochip array technology. IMA was measured by a commercial colorimetric assay on a Roche Modular system P.
Significant variations by one-way analysis of variance were observed for CK-MB (p=0.013), myoglobin (p<0.001), GPBB (p=0.029), H-FABP (p<0.001), CAIII (p=0.006), but not for cTnI (p=1.00) and IMA (p=0.881). In particular, values of all the biomarkers tested, but cTnI and IMA, increased significantly immediately after the run. GPBB and H-FABP values returned to baseline 6 and 3 h thereafter, those of CAIII, CK-MB and myoglobin remained significantly elevated from the pre-run value up to 24 h after the run. The major variation over pre-run values was recorded for myoglobin (nearly 4-fold increment), whereas CAIII, CK-MB, GPBB and H-FABP increased by 2.9-, 1.8-, 1.4- and 1.2-fold, respectively.
We conclude that a sub-maximal aerobic exercise influences the concentration of several markers of muscle damage. Except for IMA, not one of the emerging biomarkers tested can be safely used to rule out myocardial damage as well as cardiospecific troponins in patients who had undergone recent physical activity.
尽管有关于运动后肌肉和心脏损伤传统生物标志物的全面信息，但对于进行次最大运动量运动的运动员，包括缺血修饰白蛋白（IMA）、糖原磷酸化酶同工酶BB（GPBB）、碳酸酐酶III（CAIII）和心型脂肪酸结合蛋白（H-FABP）等新型标志物的动力学了解较少。
10名健康的受过训练的白种男性进行了21公里的跑步。在跑步前、跑步后即刻（post）、此后3小时、6小时和24小时采集血样。使用基于蛋白质生物芯片阵列技术的新型诊断系统检测心肌肌钙蛋白I（cTnI）、肌红蛋白、肌酸激酶同工酶MB（CK-MB）、GPBB、CAIII和H-FABP。IMA通过罗氏模块化系统P上的商业比色法进行测量。
通过单因素方差分析观察到CK-MB（p=0.013）、肌红蛋白（p<0.001）、GPBB（p=0.029）、H-FABP（p<0.001）和CAIII（p=0.006）有显著变化，但cTnI（p=1.00）和IMA（p=0.881）没有显著变化。特别是，除cTnI和IMA外，所有测试生物标志物的值在跑步后即刻均显著增加。GPBB和H-FABP的值在6小时和3小时后恢复到基线水平，CAIII、CK-MB和肌红蛋白的值在跑步后24小时内仍显著高于跑步前水平。肌红蛋白记录到相对于跑步前值的最大变化（增加近4倍），而CAIII、CK-MB、GPBB和H-FABP分别增加了2.9倍、1.8倍、1.4倍和1.2倍。
我们得出结论，次最大有氧运动影响几种肌肉损伤标志物的浓度。除IMA外，所测试的新兴生物标志物中没有一种可以安全地用于排除近期进行过体育活动的患者的心肌损伤以及心肌特异性肌钙蛋白。
