Seleem Mohamed N, Ali Mohammed, Boyle Stephen M, Sriranganathan Nammalwar
Institute for Critical Technology and Applied Science, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
FEMS Microbiol Lett. 2008 Sep;286(1):124-9. doi: 10.1111/j.1574-6968.2008.01270.x.
Despite the increasing interest in Ochrobactrum anthropi as an emerging nosocomial pathogen resistant to most commonly used antimicrobials, relatively little is known about the pathogenesis and factors contributing to its virulence. Also, many aspects of interaction between Ochrobactrum spp. and their hosts remain unclear. The ability to monitor O. anthropi infection in the host will facilitate our understanding of the pathogenic mechanisms and will lead to better choices of antimicrobial or additional therapeutic strategies. We have demonstrated the ability to stably express three reporter genes (green fluorescence protein GFP, red fluorescence protein RFP and luciferase Lux) and track the infection in a J774A.1 murine macrophage cell line as well as in the BALB/c mouse. Our results suggest that these reporter genes should improve genetic studies in O. anthropi, particularly those aimed at understanding pathogenesis, virulence factors and host interaction.
尽管嗜人苍白杆菌作为一种对最常用抗菌药物具有抗性的新兴医院病原体越来越受到关注，但对其发病机制和毒力影响因素的了解相对较少。此外，苍白杆菌属细菌与其宿主之间相互作用的许多方面仍不清楚。监测宿主中嗜人苍白杆菌感染的能力将有助于我们理解致病机制，并有助于更好地选择抗菌药物或其他治疗策略。我们已经证明能够稳定表达三种报告基因（绿色荧光蛋白GFP、红色荧光蛋白RFP和荧光素酶Lux），并在J774A.1小鼠巨噬细胞系以及BALB/c小鼠中追踪感染情况。我们的结果表明，这些报告基因应能改善对嗜人苍白杆菌的遗传学研究，特别是那些旨在了解发病机制、毒力因子和宿主相互作用的研究。
