Murray E E, Rocheleau T, Eberle M, Stock C, Sekar V, Adang M
Agrigenetics Advanced Research Corporation, Madison, WI 53716.
Plant Mol Biol. 1991 Jun;16(6):1035-50. doi: 10.1007/BF00016075.
We have examined expression of several insecticidal crystal protein (ICP) genes of Bacillus thuringiensis in transgenic tobacco plants and electroporated carrot protoplasts. We determined that low levels of lepidopteran toxin cryIA(b) ICP gene expression in plants and electroporated carrot cells is due to RNA instability. We used a series of 3' deleted by cryIA(b) constructs directed by the cauliflower mosaic virus 35S promoter to demonstrate that this instability is minimally contained in the first 579 bases of the gene in both systems. This instability may result from 5'----3' as well as 3'----5' RNA metabolism. The coleopteran toxic cryIIIA gene was also examined in electroporated carrot cells, and found to be poorly expressed. A model for improvement of ICP RNA stability in plants is presented.
我们检测了苏云金芽孢杆菌几种杀虫晶体蛋白（ICP）基因在转基因烟草植株和电穿孔胡萝卜原生质体中的表达情况。我们确定，植物和电穿孔胡萝卜细胞中鳞翅目毒素cryIA(b) ICP基因的低水平表达是由于RNA不稳定所致。我们使用了一系列由花椰菜花叶病毒35S启动子指导的cryIA(b)构建体，其3'端缺失，以证明在这两个系统中，这种不稳定性最小程度地存在于该基因的前579个碱基中。这种不稳定性可能是由5'→3'以及3'→5'的RNA代谢导致的。我们还检测了鞘翅目毒性cryIIIA基因在电穿孔胡萝卜细胞中的表达情况，发现其表达不佳。本文提出了一个提高植物中ICP RNA稳定性的模型。
