Turk S C, Melchers L S, den Dulk-Ras H, Regensburg-Tuïnk A J, Hooykaas P J
Clusius Laboratory, Leiden University, Netherlands.
Plant Mol Biol. 1991 Jun;16(6):1051-9. doi: 10.1007/BF00016076.
The induction of vir gene expression in different types of Agrobacterium strains shows different pH sensitivity profiles. The pH sensitivity pattern demonstrated by octopine Ti strains was similar to that of a supervirulent leucinopine Ti strain, whereas this was different from that shown by nopaline Ti strains and agropine Ri strains. Data are given which indicate that these differences are due to different properties of the virA genes of these wild types. An exceptional case was formed by strains with the limited-host-range plasmid pTiAG57 which showed AS-dependent vir induction only if reduced inoculum sizes were used and the temperature was 28 degrees C or below.
不同类型的农杆菌菌株中vir基因表达的诱导表现出不同的pH敏感性特征。章鱼碱型Ti菌株所表现出的pH敏感性模式与一种超毒力的亮氨酸碱型Ti菌株相似，而这与胭脂碱型Ti菌株和农杆碱型Ri菌株所表现出的模式不同。所给出的数据表明，这些差异是由于这些野生型的virA基因具有不同特性所致。具有有限宿主范围质粒pTiAG57的菌株构成了一个特殊情况，只有在使用减少的接种量且温度为28摄氏度或更低时，它们才表现出依赖于乙酰丁香酮的vir诱导。
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