Cui Jing, Wang Jie, Wang Zhong-Quan, Niu Hong-Tao
Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2008 Feb 28;26(1):73-4.
Seventy male mice (Kunming strain) were randomly divided into 7 groups (10 mice per group), each mouse was orally inoculated with 30, 25, 20, 15, 10, 5 or 3 muscle stage larvae of Trichinella spiralis, respectively. All infected mice were sacrificed 6 weeks post-inoculation, number of larvae per gram (LPG) of diaphragm were counted by compression method (trichinelloscopy), the carcass was digested by artificial digestion method and LPG was counted. The larval detection rate by trichinelloscopy and digestion method was 100% (10/10) in all mice infected with 30, 25, 20, 15 or 10 larvae, but 70% (7/10) and 100% in mice infected with 5 larvae, respectively. No larva was found by either method in mice infected with 3 larvae. There is a positive correlation between the larval burden (of diaphragm and muscle) and the infecting dose (r = 0.759, P < 0.05; r = 0.638, P < 0.05), and the minimum infection dose to a mouse is 5 larvae.
将70只雄性昆明种小鼠随机分为7组（每组10只），分别给每只小鼠经口接种30、25、20、15、10、5或3条旋毛虫肌幼虫。接种后6周处死所有感染小鼠，采用压片法（旋毛虫镜检法）计数膈肌每克幼虫数（LPG），并对鼠尸采用人工消化法消化后计数LPG。在接种30、25、20、15或10条幼虫的所有小鼠中，旋毛虫镜检法和消化法的幼虫检出率均为100%（10/10），但接种5条幼虫的小鼠中，旋毛虫镜检法和消化法的幼虫检出率分别为70%（7/10）和100%。接种3条幼虫的小鼠两种方法均未检出幼虫。膈肌和肌肉的幼虫负荷与感染剂量呈正相关（r = 0.759，P < 0.05；r = 0.638，P < 0.05），小鼠的最小感染剂量为5条幼虫。
