葡萄扇叶病毒（一种线虫传多面体病毒）蛋白在兔网织红细胞裂解物中的合成与加工

The synthesis and processing of the nepovirus grapevine fanleaf virus proteins in rabbit reticulocyte lysate.

作者信息

Morris-Krsinich B A, Forster R L, Mossop D W

机构信息

Plant Diseases Division, Department of Scientific and Industrial Research, Private Bag, Auckland, New Zealand.

出版信息

Virology. 1983 Oct 30;130(2):523-6. doi: 10.1016/0042-6822(83)90105-8.

DOI:10.1016/0042-6822(83)90105-8

PMID:18639155

Abstract

Translation of a mixture of the two grapevine fanleaf virus (GFLV) RNAS in reticulocyte lysates in the presence of the amino acid analogs canavanine, S-aminoethyl-cysteine, and p-fluorophenylalanine gave two products of molecular weights (Mr) 220,000 (from RNA-1) and 125,000 (from RNA-2). In the absence of these analogs a protease apparently induced by RNA-1 catalyzed cleavage of the RNA-2 product (Mr 125,000) into two proteins of Mr 68,000 and 58,000. The 58,000 Mr protein was shown to be the likely virus coat protein by peptide mapping after partial proteolysis. Cleavage of the RNA-2 product of GFLV was not catalyzed by the translation products of another nepovirus, tobacco ringspot virus.

摘要

在存在氨基酸类似物刀豆氨酸、S-氨乙基半胱氨酸和对氟苯丙氨酸的情况下，在网织红细胞裂解物中对两种葡萄扇叶病毒（GFLV）RNA的混合物进行翻译，得到了分子量（Mr）分别为220,000（来自RNA-1）和125,000（来自RNA-2）的两种产物。在不存在这些类似物的情况下，一种显然由RNA-1诱导的蛋白酶催化RNA-2产物（Mr 125,000）裂解为两种分子量分别为68,000和58,000的蛋白质。经部分蛋白酶解后的肽图分析表明，Mr 58,000的蛋白质可能是病毒外壳蛋白。GFLV的RNA-2产物的裂解不是由另一种线虫传多面体病毒烟草环斑病毒的翻译产物催化的。