Zhang Baowei, Maniatis Theodore, Song Yan, Zhang Wenzhong, Zhang Xiaopeng, Li Ning, Chen Junshi, Wong Andrea W, Roberts Ashley
Nucro-Technics, Scarborough, Ontario, Canada.
Mutat Res. 2008 Jul 31;654(2):133-7. doi: 10.1016/j.mrgentox.2008.05.009. Epub 2008 Jul 17.
Magnolia bark extract (MBE) has been used historically in traditional Chinese and Japanese medicines, and more recently as a component of dietary supplements and cosmetic products. The genotoxic potential of MBE was studied in two in vitro chromosomal aberration assays. In Chinese hamster ovary (CHO) cells, exposure for 3 h to MBE at concentrations of 0-30 microg/ml in the absence of a metabolic activation system (S9) and 0-7 microg/ml with S9 did not induce chromosomal aberrations, whereas higher concentrations were cytotoxic and did not allow for analysis of aberrations. Extended exposure for 18 h without metabolic activation at concentrations up to 15 microg/ml also resulted in a negative response. In V79 cells derived from Chinese hamster lung tissue, treatment for 6h with concentrations up to 52 and 59 microg/ml in the absence and presence of S9, respectively, did not increase the incidence of chromosomal aberrations compared to negative controls. Furthermore, MBE exposure for 24 h without metabolic activation did not induce aberrations. The results of these studies demonstrate that MBE is not genotoxic under the conditions of the in vitro chromosomal aberration assays in CHO and V79 cells, and support the safety of MBE.
厚朴树皮提取物（MBE）在传统的中国和日本医药中已有历史应用，最近还作为膳食补充剂和化妆品的成分。在两项体外染色体畸变试验中研究了MBE的遗传毒性潜力。在中国仓鼠卵巢（CHO）细胞中，在无代谢活化系统（S9）的情况下，暴露于浓度为0 - 30微克/毫升的MBE 3小时以及在有S9的情况下暴露于浓度为0 - 7微克/毫升的MBE 3小时，均未诱导染色体畸变，而更高浓度具有细胞毒性，无法进行畸变分析。在高达15微克/毫升的浓度下，无代谢活化的18小时延长暴露也产生了阴性反应。在源自中国仓鼠肺组织的V79细胞中，在无S9和有S9的情况下，分别用高达52和59微克/毫升的浓度处理6小时，与阴性对照相比，染色体畸变发生率并未增加。此外，无代谢活化的24小时MBE暴露未诱导畸变。这些研究结果表明，在CHO和V79细胞的体外染色体畸变试验条件下，MBE没有遗传毒性，并支持MBE的安全性。
