Gardan Delphine, Mourot Jacques, Louveau Isabelle
INRA, UMR 1079, F-35000 Rennes, France.
Mol Cell Endocrinol. 2008 Sep 24;292(1-2):63-8. doi: 10.1016/j.mce.2008.06.015. Epub 2008 Jul 2.
IGF-I and IGF-II are known to regulate cell development and recent data suggest a possible role of IGF-II on adipose tissue development. This study was undertaken to examine the IGF system gene expression in porcine differentiating adipocytes. Both adipocytes and stromal-vascular (s/v) cells were isolated from subcutaneous adipose tissue collected from 7-day-old piglets. s/v cells were cultured in chemically defined medium. Compared with isolated adipocytes, IGF-II and IGFBP-5 mRNA levels were very high in freshly isolated s/v cells, whereas IGF-I mRNA levels were lower in s/v cells than in adipocytes. Between day 0 and day 6 of culture, IGF-II and IGFBP-5 gene expression decreased whereas expression levels of late markers of adipocyte differentiation were up-regulated. Cell differentiation was also associated with an increase in the expression of IGF-I, insulin and IGF receptor genes. The current findings suggest that IGF-I and IGF-II have different effects on porcine adipose cell development.
已知胰岛素样生长因子-I（IGF-I）和胰岛素样生长因子-II（IGF-II）可调节细胞发育，最近的数据表明IGF-II在脂肪组织发育中可能发挥作用。本研究旨在检测猪分化脂肪细胞中IGF系统基因的表达。脂肪细胞和基质血管（s/v）细胞均从7日龄仔猪的皮下脂肪组织中分离得到。s/v细胞在化学成分确定的培养基中培养。与分离出的脂肪细胞相比，新鲜分离的s/v细胞中IGF-II和IGFBP-5 mRNA水平非常高，而s/v细胞中IGF-I mRNA水平低于脂肪细胞。在培养的第0天至第6天之间，IGF-II和IGFBP-5基因表达下降，而脂肪细胞分化晚期标志物的表达水平上调。细胞分化还与IGF-I、胰岛素和IGF受体基因表达的增加有关。目前的研究结果表明，IGF-I和IGF-II对猪脂肪细胞发育有不同影响。
