Batista Carlos Augusto Marques, Colleoni Neto Ramiro, Lopes Filho Gaspar de Jesus
School of Medicine of Valença, Rio de Janeiro, Brazil.
Acta Cir Bras. 2008 Jul-Aug;23(4):352-63. doi: 10.1590/s0102-86502008000400010.
To investigate the healing process of the aponeurosis of the anterior abdominal wall of rats, comparing two different materials for wound closure: 3-0 nylon suture and tissue adhesive N-butyl-2-cyanoacrylate.
Forty-four Wistar rats were randomly divided into four groups according to the type of material used (suture or adhesive) and the number of days until reoperation (seven or 14 days). After a 4 cm incision in the aponeurosis, 22 rats underwent wound closure using 3-0 nylon suture and the other 22, the tissue adhesive. After seven days, 11 rats from each group were weighed again, submitted to reoperation and then euthanized. The same procedure was carried out after 14 days with the remaining rats. The surgical wound was macroscopically examined, the tensile strength was measured and the tissue edges were histologically examined. The statistical analysis was performed using analysis of variance and Cox's proportional hazards model. Significance level was set at p < 0.05.
The animals lost on average 20 g over the period between the two operations. Wound closure was faster using the tissue adhesive. Only one animal, from the tissue adhesive group, had a small abscess with wound dehiscence. With regard to tensile strength, the best results were obtained with the tissue adhesive 14 days after the first surgery. The results of the histological examination showed no significant difference between groups.
Upon morphological evaluation, the two types of material analyzed in this study (3-0 nylon suture and N-butyl-2-cyanoacrylate, a tissue adhesive) were not significantly different with regard to the healing process of the aponeurosis of the anterior abdominal wall of rats. Wound closure using the tissue adhesive was faster. Higher tensile strength was observed in the tissue adhesive group 14 days after the first surgery.
研究大鼠前腹壁腱膜的愈合过程，比较两种不同的伤口闭合材料：3-0尼龙缝线和组织粘合剂N-丁基-2-氰基丙烯酸酯。
44只Wistar大鼠根据所用材料类型（缝线或粘合剂）和再次手术前的天数（7天或14天）随机分为四组。在腱膜上做一个4厘米的切口后，22只大鼠用3-0尼龙缝线进行伤口闭合，另外22只用组织粘合剂。7天后，每组11只大鼠再次称重，接受再次手术，然后安乐死。14天后对其余大鼠进行相同操作。对手术伤口进行宏观检查，测量拉伸强度，并对组织边缘进行组织学检查。使用方差分析和Cox比例风险模型进行统计分析。显著性水平设定为p < 0.05。
在两次手术期间，动物平均体重减轻20克。使用组织粘合剂伤口闭合更快。只有组织粘合剂组的一只动物出现了小脓肿并伴有伤口裂开。关于拉伸强度，首次手术后14天使用组织粘合剂获得了最佳结果。组织学检查结果显示各组之间无显著差异。
在形态学评估中，本研究分析的两种材料（3-0尼龙缝线和组织粘合剂N-丁基-2-氰基丙烯酸酯）在大鼠前腹壁腱膜的愈合过程方面没有显著差异。使用组织粘合剂伤口闭合更快。首次手术后14天，组织粘合剂组观察到更高的拉伸强度。
