Damous Luciana Lamarão, da Silva Sônia Maria, Simões Ricardo Dos Santos, Sakano Célia Regina de Souza Bezerra, Simões Manuel de Jesus, Montero Edna Frasson de Souza
Surgery and Research Post-Graduate Program, UNIFESP, São Paulo, Brazil.
Acta Cir Bras. 2008 Jul-Aug;23(4):372-7. doi: 10.1590/s0102-86502008000400012.
To morphologically evaluate the estrogenic effect on the uterus and vagina of rats submitted to ovarian autografts.
Twenty Wistar EPM-1 adult rats were bilaterally ovariectomized, followed by ovarian transplants in retroperitoneal regions. The animals were divided in four groups of five animals, according to the day of euthanasia: G4, G7, G14 and G21, corresponding to the 4th, 7th, 14th and 21st day after surgery, respectively. Vaginal smears were collected from the first day of surgery until euthanasia day. After that, the vagina and uterus were removed, fixed in 10% formaldehyde and submitted to histological analysis and stained with hematoxiline and eosine.
All animals showed estrous cycle changes during the experiment. In 4th day, the uterus showed low action of estrogen with small number of mitosis and eosinophils as well as poor development. On the 7th day, the endometrium was atrophic without mitotic signals and presented a small number of eosinophils. On the 14th and 21th days the histological findings were similar, with the presence of mitosis in the endometrial glands and intense leucocyte infiltration with a large number of eosinophils. Morphometric results showed that the endometrial and myometrial thickness as well as the number of eosinophils presented the highest values during the 14th and 21th days of the evaluation. The 7th day group also presented the lowest eosinophil numbers. Vaginal epithelium features were: 4th and 7th day groups presented non-keratinized stratified epithelium with 5 and 2 cell layers, respectively. The 14th and 21st day groups presented non-keratinized stratified epithelium with 14 and 15 cell layers.
Experimental ovarian autografts in the evaluated organs presented maximum estrogen activity after the 21st day of surgery, according to morphological and morphometric data.
从形态学上评估卵巢自体移植对大鼠子宫和阴道的雌激素作用。
将20只成年Wistar EPM - 1大鼠双侧卵巢切除，随后在腹膜后区域进行卵巢移植。根据安乐死时间将动物分为四组，每组五只：G4、G7、G14和G21，分别对应术后第4天、第7天、第14天和第21天。从手术第一天至安乐死日收集阴道涂片。之后，取出阴道和子宫，用10%甲醛固定，进行组织学分析，并用苏木精和伊红染色。
所有动物在实验期间均出现动情周期变化。第4天，子宫显示雌激素作用较弱，有丝分裂和嗜酸性粒细胞数量少，发育不良。第7天，子宫内膜萎缩，无有丝分裂信号，仅有少量嗜酸性粒细胞。第14天和第21天的组织学结果相似，子宫内膜腺体有有丝分裂，大量嗜酸性粒细胞浸润明显。形态计量学结果显示，在评估的第14天和第21天，子宫内膜和肌层厚度以及嗜酸性粒细胞数量达到最高值。第7天组的嗜酸性粒细胞数量也最低。阴道上皮特征为：第4天和第7天组分别呈现非角化复层上皮，细胞层数为5层和2层。第14天和第21天组呈现非角化复层上皮，细胞层数为14层和15层。
根据形态学和形态计量学数据，在所评估器官中进行的实验性卵巢自体移植在术后第21天表现出最大雌激素活性。
