Stocker Hugo, Gallant Peter
Institute for Molecular Systems Biology, ETH Zürich, Zürich, Switzerland.
Methods Mol Biol. 2008;420:27-44. doi: 10.1007/978-1-59745-583-1_2.
Drosophila melanogaster has long been a prime model organism for developmental biologists. During their work, they have established a large collection of techniques and reagents. This in turn has made fruit flies an attractive system for many other biomedical researchers who have otherwise no background in fly biology. This review intends to help Drosophila neophytes in setting up a fly lab. It briefly introduces the biological properties of fruit flies, describes the minimal equipment required for working with flies, and offers some basic advice for maintaining fly lines and setting up and analyzing experiments.
黑腹果蝇长期以来一直是发育生物学家的主要模式生物。在他们的工作过程中,已经建立了大量的技术和试剂。这反过来又使果蝇成为许多其他生物医学研究人员感兴趣的系统,而这些研究人员原本没有果蝇生物学背景。这篇综述旨在帮助果蝇新手建立果蝇实验室。它简要介绍了果蝇的生物学特性,描述了研究果蝇所需的最少设备,并提供了一些关于维持果蝇品系以及设置和分析实验的基本建议。