在. 中测量反刍进食反应
Measurement of Reflexive Feeding Response in .
机构信息
Department of Biology, Texas A&M University, College Station, Texas 77843, USA.
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.
出版信息
Cold Spring Harb Protoc. 2023 Jun 1;2023(6):pdb.prot108092. doi: 10.1101/pdb.prot108092.
The ability to distinguish between food sources that are good and provide nutrients and those that are potentially dangerous is crucial to the survival of an organism. Here, we describe a taste assay that measures the reflexive feeding response to a given tastant. To examine taste preference for a soluble compound, an appetitive tastant is applied to the proboscis, and the proportion of proboscis extensions are recorded. This single-fly assay may be applied to adult of any genetic background and facilities examination of the neural circuitry and molecular processes encoding the reflexive taste response. Furthermore, this assay requires few custom components and therefore can be easily established in laboratories with minimal expertise in the study of fly behavior.
区分有益营养食物来源和潜在危险食物来源的能力对生物体的生存至关重要。在这里，我们描述了一种味觉分析方法，用于测量对特定味觉刺激的反射性摄食反应。为了检测对可溶性化合物的味觉偏好，将开胃味觉刺激物应用于喙，然后记录喙伸展的比例。这种单蝇检测法可用于任何遗传背景的成年蝇，并可用于研究编码反射性味觉反应的神经回路和分子过程。此外，这种检测方法所需的定制组件很少，因此可以很容易地在对蝇类行为研究经验有限的实验室中建立。
Zotero 插件