Sakurai A, Imai H, Takenaka Y, Sakakibara M
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Fukui 910, Japan; fax: +81-776-27-8747.
Biotechnol Bioeng. 1997 Dec 20;56(6):689-96. doi: 10.1002/(SICI)1097-0290(19971220)56:6<689::AID-BIT12>3.0.CO;2-3.
A simple model was presented to describe the time courses of citric acid production by a rotating disc contactor (RDC) using Aspergillus niger. The model is expressed by Monod-type cell growth, Luedeking-Piret-type citric acid production rate equations, and the diffusion equation for oxygen in the biofilm. The model contains five parameters which were determined by the nonlinear least squares method by fitting the numerical solution to the experimental data. In solving the equations, the cell density of the biofilm was estimated from the value of cellular mass per unit of biofilm area using an empirical equation. The experimental time courses in citric acid production period were well simulated with this model. The relation between the specific biofilm surface area and the rate of citric acid production was also explained by the simulation using the average values of five parameters of twelve runs. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 689-696, 1997.
提出了一个简单的模型来描述使用黑曲霉的转盘接触器（RDC）生产柠檬酸的时间进程。该模型由莫诺德型细胞生长、吕德金-皮雷特型柠檬酸生产率方程以及生物膜中氧气的扩散方程表示。该模型包含五个参数，这些参数通过将数值解与实验数据拟合的非线性最小二乘法确定。在求解方程时，使用经验方程根据单位生物膜面积的细胞质量值估算生物膜的细胞密度。该模型很好地模拟了柠檬酸生产期间的实验时间进程。还通过使用十二次运行的五个参数的平均值进行模拟，解释了比生物膜表面积与柠檬酸生产率之间的关系。(c) 1997 John Wiley & Sons, Inc.《生物技术与生物工程》56: 689 - 696, 1997年。
