Droller M J
Invest Urol. 1976 Nov;14(3):249-52.
Cyclic AMP may be involved in the modulation of cell growth. The present work sought to further define differences between normal cells and tumor cells in their cyclic AMP system. Mouse embryo fibroblasts and murine bladder transitional epithelium tumor cells were grown in vitro; at various times, adenyl cyclase activity was assayed by measuring the conversion of [alpha32P]ATP to cyclic AM32P; stimulation by prostaglandin E1 or sodium fluoride was also determined. Base line and fluoride-stimulated enzyme activity were significantly greater in normal cells than tumor cells (P less than 0.01), and reached a peak at day 2; at confluency, levels in both systems decreased. Prostaglandin E1-stimulated levels, in contrast, were greater in tumor cells, there being a 10 fold greater relative stimulation in these cells compared to normal cells (P less than 0.01). Findings of a possibly greater sensitivity in these tumor cells may be important in a therapeutic modulation of tumor growth.
环磷酸腺苷（cAMP）可能参与细胞生长的调节。目前的研究旨在进一步明确正常细胞和肿瘤细胞在其环磷酸腺苷系统中的差异。小鼠胚胎成纤维细胞和小鼠膀胱移行上皮肿瘤细胞在体外培养；在不同时间，通过测量[α32P]ATP转化为环AM32P来测定腺苷酸环化酶活性；同时也测定前列腺素E1或氟化钠的刺激作用。正常细胞的基础和氟化物刺激的酶活性显著高于肿瘤细胞（P<0.01），并在第2天达到峰值；汇合时，两个系统的水平均下降。相比之下，前列腺素E1刺激的水平在肿瘤细胞中更高，这些细胞中的相对刺激比正常细胞大10倍（P<0.01）。这些肿瘤细胞可能具有更高敏感性的发现，对于肿瘤生长的治疗性调节可能具有重要意义。
