Hinojosa-Juarez Araceli Consuelo, Sandoval-Balanzario Miguel, McManus Donald Peter, Monroy-Ostria Amalia
Departamento de Inmunologia, Escuela Nacional de Ciencias Biologicas, IPN, Carpio y Plan de Ayala, Col. Casco de Santo Tomas, C.P. 11340, Mexico, D.F., Mexico.
Infect Genet Evol. 2008 Sep;8(5):653-6. doi: 10.1016/j.meegid.2008.06.004. Epub 2008 Jul 3.
Mitochondrial (mt) cox1 and ribosomal ITS1 DNA sequences from Taenia solium cysticercus isolates from pigs and cysticerci (racemose and cellulose types) from patients with neurocysticercosis were amplified by the polymerase chain reaction (PCR). The amplicons were sequenced in order to determine the genetic relationship between these types of cysticerci. Phylogenetic trees were constructed and evolutionary distances were calculated. ITS1 and mt cox1 cysticerci sequence data were compared with previously published Taenia spp. sequences. The variation in the ITS1 and cox1 sequences of samples collected from Mexico was minimal, regardless of geographical origin, size or colour of cysticerci from either pigs or human brain. These results suggest that the racemose and cellulose types represent genetically identical metacestodes of T. solium. Alignment of the mt cox1 sequences of the Mexican samples with sequences of other Taenia taxa showed that most were very similar to T. solium from Mexico and T. solium from Colombia; one T. solium Mexican isolate and Taenia hydatigena were placed in the same group close to Taenia crassiceps. The ITS1 sequences for the Mexican T. solium samples indicated the majority were in the same group as the Latin American T. solium. Two Mexican T. solium samples and T. solium from Philippines were placed together in a different group.
采用聚合酶链反应（PCR）扩增了来自猪的猪带绦虫囊尾蚴分离株以及来自神经囊尾蚴病患者的囊尾蚴（葡萄状和纤维素状类型）的线粒体（mt）细胞色素c氧化酶亚基1（cox1）和核糖体内部转录间隔区1（ITS1）DNA序列。对扩增产物进行测序，以确定这些类型囊尾蚴之间的遗传关系。构建了系统发育树并计算了进化距离。将ITS1和mt cox1囊尾蚴序列数据与先前发表的带绦虫属序列进行了比较。无论猪或人脑来源的囊尾蚴的地理来源、大小或颜色如何，从墨西哥采集样本的ITS1和cox1序列变异极小。这些结果表明，葡萄状和纤维素状类型代表了猪带绦虫基因相同的中绦期幼虫。墨西哥样本的mt cox1序列与其他带绦虫类群的序列比对显示，大多数与来自墨西哥的猪带绦虫和来自哥伦比亚的猪带绦虫非常相似；一个墨西哥猪带绦虫分离株和多头带绦虫被置于与肥胖带绦虫接近的同一组中。墨西哥猪带绦虫样本的ITS1序列表明，大多数与拉丁美洲猪带绦虫属于同一组。两个墨西哥猪带绦虫样本和来自菲律宾的猪带绦虫被放在一个不同的组中。
