一种类内收蛋白在小鼠卵母细胞染色体中的免疫荧光定位。

Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes.

作者信息

Pinto-Correia C, Goldstein E G, Bennett V, Sobel J S

机构信息

Department of Anatomical Sciences, State University of New York, Buffalo 14214.

出版信息

Dev Biol. 1991 Aug;146(2):301-11. doi: 10.1016/0012-1606(91)90232-r.

DOI:10.1016/0012-1606(91)90232-r

PMID:1864459

Abstract

The mouse oocyte expresses a polypeptide of Mr 120,000 that cross-reacts with an antibody to the brain membrane skeletal protein adducin. Immunofluorescence localization showed a bright chromosomal staining reaction in metaphase I and metaphase II oocytes. Following in vitro fertilization the maternal chromosomes lost their immunoreactivity during pronuclear development. The fertilizing sperm chromatin and male pronucleus did not show any detectable staining reaction. Bright chromosomal fluorescence was again observed in the first mitotic metaphase when both maternal and paternal chromosomes gave a positive staining reaction. In contrast to the immunoreactivity of the maternal meiotic chromosomes, the meiotic chromosomes of male germ line cells failed to exhibit any detectable staining reaction and this difference was confirmed by immunolabeling of oocyte and spermatocyte karyotypes. Mitotic chromosomes in preimplantation embryos, fetal liver, adult intestinal epithelium, and MDCK cells also failed to show any detectable labeling reaction. The results suggest that expression of the immunoreactive chromosomal adducin may be a unique feature of oogenesis.

摘要

小鼠卵母细胞表达一种分子量为120,000的多肽，它能与抗脑膜骨架蛋白内收蛋白的抗体发生交叉反应。免疫荧光定位显示，在第一次减数分裂中期和第二次减数分裂中期的卵母细胞中，染色体呈现明亮的染色反应。体外受精后，母源染色体在原核发育过程中失去了免疫反应性。受精的精子染色质和雄原核未显示任何可检测到的染色反应。在第一次有丝分裂中期再次观察到明亮的染色体荧光，此时母源和父源染色体均呈阳性染色反应。与母源减数分裂染色体的免疫反应性相反，雄性生殖系细胞的减数分裂染色体未表现出任何可检测到的染色反应，卵母细胞和精母细胞核型的免疫标记证实了这种差异。植入前胚胎、胎儿肝脏、成年肠上皮和MDCK细胞中的有丝分裂染色体也未显示任何可检测到的标记反应。结果表明，免疫反应性染色体内收蛋白的表达可能是卵子发生的一个独特特征。