Latifynia Afshineh, Hazrati Saleh Mohaghegh
University of Tehran School of Medicine, Medical Sciences, Tehran, Iran.
Turkiye Parazitol Derg. 2008;32(2):103-8.
Leishmania parasites cause a spectrum of diseases that afflict the populations of 88 countries around the world and all attempts to control leishmaniasis have failed. It seems that preparing a vaccine may be the final solution. The aim of this study was to determine various Leishmania (L.) major antigens vaccine candidates and effects of the vaccine on delayed type hypersensitivity (DTH). Many different methods of vaccine preparation plus or without adjuvant were used. We prepared crude antigen combinations by five different methods using antigens from L. major parasites. Phase I was done in animals. The immunogenic effect was evaluated with the delayed type hypersensitivity (DTH) reaction with five different doses, including 100, 200, 300, 400, 500 microg/ml of total protein + BCG in Balb/c and conventional laboratory white mice (out breed). Our results showed that the cocktail antigen was highly specific. No injection of BCG solvent or saline treated controls showed significant results. Taken together, the effect of cellular immune response to the cocktail vaccine induced a significant effect against cutaneous leishmaniasis in the experimental model of vaccine with L. major.
利什曼原虫寄生虫引发了一系列疾病，困扰着全球88个国家的民众，所有控制利什曼病的尝试均告失败。制备疫苗似乎可能是最终的解决办法。本研究的目的是确定多种利什曼原虫（硕大利什曼原虫）主要抗原疫苗候选物以及该疫苗对迟发型超敏反应（DTH）的影响。使用了许多不同的制备疫苗方法，有加佐剂的和不加佐剂的。我们使用硕大利什曼原虫寄生虫的抗原，通过五种不同方法制备了粗抗原组合。第一阶段在动物身上进行。用迟发型超敏反应（DTH）评估免疫原性效应，使用五种不同剂量，包括在Balb/c小鼠和常规实验室白色小鼠（远交群）中每毫升含100、200、300、400、500微克总蛋白 + 卡介苗。我们的结果表明，混合抗原具有高度特异性。未注射卡介苗溶剂或生理盐水处理的对照组未显示出显著结果。综上所述，在硕大利什曼原虫疫苗实验模型中，细胞免疫反应对混合疫苗的作用诱导了针对皮肤利什曼病的显著效果。
