Piekarska Katarzyna, Gierczyński Rafał, Ławrynowicz-Paciorek Maja, Kochman Maria, Jagielski Marek
National Institute of Public Health, National Institute of Hygiene, Department of Bacteriology, Warsaw, Poland.
Pol J Microbiol. 2008;57(2):121-4.
The activity of ciprofloxacin, sparfloxacin and moxifloxacin was determined for 205 Enterococcus faecalis isolates from patients of five hospitals (Warsaw, Poland; collected from 2000 to 2002). Ciprofloxacin resistant and intermediate isolates were numerous (53.7%). Among them, highly resistant (MIC > or = 16 mg/l) isolates predominated (98%). Isolates resistant to ciprofloxacin were also resistant to sparfloxacin and moxifloxacin. The parC and gyrA QRDRs (quinolone-resistance-determining region) of 11 isolates with ciprofloxacin MICs from 1 to 256 mg/l were analysed by DNA sequencing. In ParC one kind of amino acid substitution (of Ser-85 to Ile) in 9 E. faecalis strains with MICs from 16 to 256 mg/l was observed. In GyrA Ser-84 was changed to one of four different amino acids: Arg, Ile, Cys or Tyr, however no association between the amino acid type and MIC value was found. The last two substitutions have not been reported to date for E. faecalis. Moreover, our results may suggest that mutations within parC and gyrA are associated with development of a high-level of ciprofloxacin resistance.
对来自五家医院（波兰华沙；2000年至2002年收集）患者的205株粪肠球菌分离株测定了环丙沙星、司帕沙星和莫西沙星的活性。对环丙沙星耐药和中介的分离株数量众多（53.7%）。其中，高度耐药（MIC≥16mg/L）的分离株占主导（98%）。对环丙沙星耐药的分离株对司帕沙星和莫西沙星也耐药。通过DNA测序分析了11株环丙沙星MIC为1至256mg/L的分离株的parC和gyrA喹诺酮耐药决定区（QRDR）。在ParC中，观察到9株MIC为16至256mg/L的粪肠球菌菌株中有一类氨基酸取代（Ser-85变为Ile）。在GyrA中，Ser-84变为四种不同氨基酸之一：Arg、Ile、Cys或Tyr，然而未发现氨基酸类型与MIC值之间存在关联。后两种取代迄今为止尚未在粪肠球菌中报道。此外，我们的结果可能表明parC和gyrA内的突变与高水平环丙沙星耐药性的发展有关。
