Wolska Katarzyna, Szweda Piotr
Department of Microbiology, Agricultural Faculty, University of Podlasie, Siedlce, Poland.
Pol J Microbiol. 2008;57(2):157-63.
Two typing methods were evaluated, utilizing 62 clinical strains of Pseudomonas aeruginosa, to assess their usefulness as tools to study the bacterial diversity within this complex group. Genetic diversity was determined by PCR ribotyping and enterobacterial repetitive intergenic consensus (ERIC) PCR. By these methods, 9 and 36 genotypes were found, respectively. The result showed that ERIC PCR analysis is a more discriminatory method than PCR ribotyping analysis and traditional serotyping scheme. We suggest that maximum discrimination can be achievied by a combination of these methods.
利用62株铜绿假单胞菌临床菌株评估了两种分型方法，以评估它们作为研究这一复杂菌群内细菌多样性工具的实用性。通过聚合酶链反应（PCR）核糖体分型和肠杆菌基因间重复共有序列（ERIC）PCR确定遗传多样性。通过这些方法，分别发现了9种和36种基因型。结果表明，ERIC PCR分析比PCR核糖体分型分析和传统血清分型方案更具鉴别力。我们建议，将这些方法结合使用可实现最大程度的鉴别。
