[苯并[a]芘二醇环氧化物诱导的转化细胞中Ras家族基因表达的研究]

[Study on expression of Ras family gene in transformed cells induced by BPDE].

作者信息

Zhou Lanlan, Tan Aijun, Jiang Yiguo, Shen Yuelan

机构信息

Institute for Chemical Carcinogenesis, Department of Preventive Medicine, Guangzhou Medical College, Guangzhou 510182, China.

出版信息

Wei Sheng Yan Jiu. 2008 May;37(3):259-61.

PMID:18646517

Abstract

OBJECTIVE

To explore the expression of Ras family gene in the malignantly transformed human bronchial epithelial cell line induced by anti-benzo(a)pyrene-trans-7,8- dihydrodiol-9,10-epoxide(BPDE).

METHODS

The levels of H-Ras, K-Ras and N-Ras mRNA expression in BPDE transformed 16HBE cells (16HBE-T) and untransformed control 16HBE cells (16HBE-N) were examined using RT-PCR. Expression levels of those three proteins in both kinds of cells were analyzed by western blot and immunocytochemical method.

RESULTS

Compared with 16HBE-N, the levels of H-Ras, K-Ras and N-Ras mRNA expression were significantly increased to 2.237, 1.254 and 3.616 times in 16HBE-T, and the protein levels of those genes were increased to 1.273, 1.547 and 1.600 times, respectively. Immunocytochemical method showed expression levels of those proteins in 16HBE-T were significantly higher than those in 16HBE-N.

CONCLUSION

H-Ras, K-Ras and N-Ras are overexpressed in 16HBE-T. The activation of oncogenic Ras may participate in malignant transformation of 16HBE induced by BPDE.

摘要

目的

探讨抗苯并（a）芘-反式-7,8-二氢二醇-9,10-环氧化物（BPDE）诱导的人支气管上皮细胞恶性转化株中Ras家族基因的表达情况。

方法

采用逆转录-聚合酶链反应（RT-PCR）检测BPDE转化的16HBE细胞（16HBE-T）及未转化的对照16HBE细胞（16HBE-N）中H-Ras、K-Ras和N-Ras mRNA的表达水平。通过蛋白质印迹法和免疫细胞化学方法分析两种细胞中这三种蛋白的表达水平。

结果

与16HBE-N相比，16HBE-T中H-Ras、K-Ras和N-Ras mRNA的表达水平显著升高，分别为2.237倍、1.254倍和3.616倍，这三种基因的蛋白水平分别升高至1.273倍、1.547倍和1.600倍。免疫细胞化学方法显示，16HBE-T中这些蛋白的表达水平显著高于16HBE-N。