[抗苯并[a]芘二醇环氧化物对人支气管上皮细胞系中HER2/neu基因表达的影响]

[Effect of anti-BPDE on expression of HER2/neu gene in the human bronchial epithelial cell line].

作者信息

Fu Juan, Jiang Yiguo, Zou Yunfeng, Shen Yuelan

机构信息

Department of Occupational and Environmental Health, MOE Key Lab of Environment and Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Wei Sheng Yan Jiu. 2007 Nov;36(6):657-9.

PMID:18303620

Abstract

OBJECTIVE

To explore the effects of anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (anti-BPDE) on expression of HER2/neu gene in the human bronchial epithelial cells.

METHODS

The levels of HER2/neu mRNA and protein expressions, and its protein localization were examined in the malignant transformed 16HBE (16HBE-T) induced by 2.0 micromol/L anti-BPDE and untransformed control 16HBE (16HBE-N) using semi-quantitative RT-PCR, SYBR Green I Real time quantitative RT-PCR (QRT-PCR), western blot and immunocytochemical method respectively.

RESULTS

The expressions of mRNA and protein of HER2/neu appeared to be a significant increase in 16HBE-T in comparison with those of 16HBE-N detected by several assays. HER2/neu protein showed membrane localization.

CONCLUSION

HER2/neu could be overexpressed on treatment of human bronchial epithelial cells with anti-BPDE. The activity of HER2/neu oncogene might play an important role in the malignant transformed 16HBE induced by anti-BPDE.

摘要

目的

探讨抗苯并（a）芘-反式-7,8-二氢二醇-9,10-环氧化物（anti-BPDE）对人支气管上皮细胞中HER2/neu基因表达的影响。

方法

分别采用半定量逆转录-聚合酶链反应（RT-PCR）、SYBR Green I实时定量逆转录-聚合酶链反应（QRT-PCR）、蛋白质免疫印迹法和免疫细胞化学方法，检测2.0微摩尔/升anti-BPDE诱导的恶性转化16HBE细胞（16HBE-T）和未转化的对照16HBE细胞（16HBE-N）中HER2/neu mRNA和蛋白表达水平及其蛋白定位。