Chardin H, Septier D, Goldberg M
Laboratoire d'Histologie, Biologie et Biominéralisation des matrices extracellulaires, Faculté de Chirurgie Dentaire, Montrouge, France.
J Biol Buccale. 1991 Mar;19(1):99-105.
In the present study, antibodies against rat dental proteoglycans were used to characterize and localize the proteoglycans in rat incisor and mandibular tissues. Polyclonal rabbit antibodies were raised against a CPC-precipitated fraction of a sulfated dental extract. In unpurified dental extract these antibodies recognized two molecules of 110 kD and 150 kD. The 150 kD molecule was susceptible to chondroitinase ABC digestion but the 110 kD molecule resisted this enzymatic degradation. Immunocytochemically these two molecules were seen to be located in the pulp, the enamel organ and the mandibular bone. In each tissue only the periphery of the cells was stained and not the intracellular compartment. In the mineralized area of bone, dentin and forming enamel no staining was seen. These results indicate common epitopes in the proteoglycans from pulp, predentin, enamel organ and bone. Some differences were found in the nature of tooth and bone proteoglycans.
在本研究中，使用抗大鼠牙齿蛋白聚糖的抗体来表征和定位大鼠切牙及下颌组织中的蛋白聚糖。针对硫酸化牙齿提取物的CPC沉淀部分制备了多克隆兔抗体。在未纯化的牙齿提取物中，这些抗体识别出110 kD和150 kD的两种分子。150 kD的分子对软骨素酶ABC消化敏感，但110 kD的分子抵抗这种酶促降解。免疫细胞化学显示这两种分子位于牙髓、釉质器和下颌骨中。在每个组织中，仅细胞周边被染色，细胞内区域未被染色。在骨、牙本质和正在形成的釉质的矿化区域未观察到染色。这些结果表明牙髓、前期牙本质、釉质器和骨中的蛋白聚糖具有共同的表位。在牙齿和骨蛋白聚糖的性质上发现了一些差异。
