Hamblin C, Mertens P P, Mellor P S, Burroughs J N, Crowther J R
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, U.K.
J Virol Methods. 1991 Feb-Mar;31(2-3):285-92. doi: 10.1016/0166-0934(91)90166-w.
A serogroup specific, indirect, sandwich ELISA was developed for the rapid detection of African horse sickness virus and viral antigens in field samples or in infected tissue cultures. The assay was shown to be highly sensitive and capable of providing confirmation of clinical diagnosis within one day. The results demonstrated that this ELISA will be useful for epidemiological surveillance of insect and mammalian host populations.
开发了一种血清群特异性间接夹心ELISA，用于快速检测野外样本或受感染组织培养物中的非洲马瘟病毒和病毒抗原。该检测方法显示出高度敏感性，能够在一天内为临床诊断提供确认。结果表明，这种ELISA将有助于对昆虫和哺乳动物宿主种群进行流行病学监测。
