Wang Li-Hong, Zhou Qing, Zeng Qing-Ling
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Huan Jing Ke Xue. 2008 Mar;29(3):799-803.
Responses of sugar metabolism during germination of rice (O. sativa ), wheat (T. aestivum) and rape (B. chinensis var. oleifera) seeds to simulated acid rain (pH 2.0, pH 2.5, pH 3.0, pH 3.5, pH 4.0, pH 4.5, pH 5.0) were investigated. The purpose was to clarify the mechanism of acid rain affecting seed germination. The results show that the alpha-amylase activity, contents of soluble sugar and reducing sugar of the rice, wheat and rape seeds decrease with increased stress level (pH 5.0 - 2.0), and are lower than CK. The response order of three indexes to stress level of acid rain is that rice (pH 3.5 - 4.0/53.88% - 77.7%) is smaller than wheat (pH 3.5 - 4.5/58.60% - 89.41%), and rape (pH 4.0 - 5.0/60.14% - 100%) is the smallest, alpha-amylase activity, contents of soluble sugar and reducing sugar of rice increase with prolonged stress time, but the three indexes of wheat and rape increase at first, and then decrease. In the same stress time (3 - 7 d), the three indexes of the three species for all treatment groups are lower than CK, and decrease with increased stress level. The stress time when the maximum damage of a-amylase activity, contents of soluble sugar and reducing sugar appeared is that rice (7 d, 7 d, 7 d) > wheat (7 d, 6 d, 5 d) > rape (3 d, 7 d, 5 d). Responses of three indexes to stress level and stress time of acid rain show that the ability of sugar metabolism resisting acid rain is that rice is stronger than wheat and rape is the worst, and the difference in sugar metabolism of 3 species is one of the internal reasons why the germination indexes behave differently.
研究了水稻（O. sativa）、小麦（T. aestivum）和油菜（B. chinensis var. oleifera）种子萌发过程中糖代谢对模拟酸雨（pH 2.0、pH 2.5、pH 3.0、pH 3.5、pH 4.0、pH 4.5、pH 5.0）的响应。目的是阐明酸雨影响种子萌发的机制。结果表明，水稻、小麦和油菜种子的α-淀粉酶活性、可溶性糖和还原糖含量随胁迫水平升高（pH 5.0 - 2.0）而降低，且低于对照。三个指标对酸雨胁迫水平的响应顺序为：水稻（pH 3.5 - 4.0/53.88% - 77.7%）小于小麦（pH 3.5 - 4.5/58.60% - 89.41%），油菜（pH 4.0 - 5.0/60.14% - 100%）最小；水稻的α-淀粉酶活性、可溶性糖和还原糖含量随胁迫时间延长而增加，但小麦和油菜的这三个指标先增加后降低。在相同胁迫时间（3 - 7 d）内，所有处理组三种作物的这三个指标均低于对照，且随胁迫水平升高而降低。α-淀粉酶活性、可溶性糖和还原糖出现最大损伤时的胁迫时间为：水稻（7 d、7 d、7 d）＞小麦（7 d、6 d、5 d）＞油菜（3 d、7 d、5 d）。三个指标对酸雨胁迫水平和胁迫时间的响应表明，糖代谢抵抗酸雨的能力为水稻强于小麦，油菜最差，三种作物糖代谢的差异是萌发指标表现不同的内在原因之一。
