γ干扰素降低前列腺癌细胞的黏附性和侵袭性
[Interferon-gamma decreases the adhesiveness and invasiveness of prostate cancer cells].
作者信息
Gao Lin, Yang De-jun, Lin Xiang-guo, Xu Wan-hai, Wang Xiao-min
机构信息
Department of Urology, the Fourth Hospital Affiliated to Harbin Medical University, Harbin, Heilongjiang 150001, China.
出版信息
Zhonghua Nan Ke Xue. 2008 Jun;14(6):513-6.
OBJECTIVE
To study the effects of interferon-gamma (IFN-gamma) on the adhesiveness and invasiveness of prostate cancer cells.
METHODS
We determined the effect of IFN-gamma on the adhesiveness of prostate cancer cell lines LNCaP and PC-3 by treating the plate with fibronectin and laminin, on the invasiveness of prostate cancer cells into the artificial basal membrane consisting of Matrigel and fibronectin using the Transwell chamber, and on the expression of annexin-2 in these cells by Western blot.
RESULTS
The adhesion rates of prostate cancer cell lines LNCaP and PC-3 were 21% and 23% in the IFN-gamma treatment group, significantly lower than 46% and 40% in the untreated group (P < 0.05). The invasiveness of the cells was markedly decreased in the former as compared with the latter (P < 0.05). IFN-gamma significantly inhibited the expression of annexin-2 in prostate cancer cell lines LNCaP and PC-3 (P < 0.05).
CONCLUSION
IFN-gamma can decrease the adhesiveness and invasiveness of the prostate cancer cell line by down-regulating the expression of annexin-2.
目的
研究γ干扰素（IFN-γ）对前列腺癌细胞黏附性和侵袭性的影响。
方法
我们通过用纤连蛋白和层粘连蛋白处理培养板来测定IFN-γ对前列腺癌细胞系LNCaP和PC-3黏附性的影响，使用Transwell小室测定前列腺癌细胞对由基质胶和纤连蛋白组成的人工基底膜的侵袭性，并通过蛋白质印迹法检测这些细胞中膜联蛋白-2的表达。
结果
IFN-γ处理组中前列腺癌细胞系LNCaP和PC-3的黏附率分别为21%和23%，显著低于未处理组的46%和40%（P<0.05）。与后者相比，前者细胞的侵袭性明显降低（P<0.05）。IFN-γ显著抑制前列腺癌细胞系LNCaP和PC-3中膜联蛋白-2的表达（P<0.05）。
结论
IFN-γ可通过下调膜联蛋白-2的表达来降低前列腺癌细胞系的黏附性和侵袭性。
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