Liu Bo, Liu Ji-hong, Wang Tao, Yang Jun, Wang Shao-gang, Lan Ru-zhu, Ye Zhang-qun
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Zhonghua Nan Ke Xue. 2008 Jun;14(6):524-6.
To investigate the effects of testosterone on the proliferation of penile corpus cavernosal cells in male SD rats.
Smooth muscle cells (SMCs) and fibroblasts collected from the corpus cavernosal tissues of male SD rats were cultured by the enzymatic dispersion method and detected for the expression of the androgen receptor (AR) by immunohistochemistry. The effects of testosterone on the SMCs and fibroblasts were observed by methyl thiazolyl tetrazolium (MTT) assay in different concentration groups (10(-8) mol/L, 10(-7) mol/L, 10(-6) mol/L, 10(-5) mol/L, 10(-4) mol/L and 10(-3) mol/L) in comparison with the control.
The AR expression was found in the penile corpus cavernosal tissues. MTT assay showed that, at the concentration of 10(-5) mol/L, testosterone induced the proliferation of SMCs (68100 +/- 2200) and fibroblasts (70200 +/- 1300), with significant differences from the control ( P < 0.05), while at 10(-4) mol/L, it inhibited their proliferation (55000 +/- 1400 and 59100 +/- 1500, respectively), (P < 0.01). No significant effects were noted in the other concentration groups.
AR exists in the penile corpus cavernosal tissues of male rats. Testosterone modulates the proliferation of corpus cavernosum tissue cells through AR, and different concentrations of testosterone may be positively or negatively correlated with the proliferation of SMCs and fibroblasts.
探讨睾酮对雄性SD大鼠阴茎海绵体细胞增殖的影响。
采用酶消化法培养雄性SD大鼠阴茎海绵体组织中的平滑肌细胞（SMCs）和成纤维细胞，免疫组织化学法检测雄激素受体（AR）的表达。采用甲基噻唑基四氮唑（MTT）法观察不同浓度组（10⁻⁸mol/L、10⁻⁷mol/L、10⁻⁶mol/L、10⁻⁵mol/L、10⁻⁴mol/L和10⁻³mol/L）睾酮对SMCs和成纤维细胞的影响，并与对照组比较。
阴茎海绵体组织中存在AR表达。MTT法显示，在10⁻⁵mol/L浓度时，睾酮诱导SMCs（68100±2200）和成纤维细胞（70200±1300）增殖，与对照组相比差异有统计学意义（P<0.05）；而在10⁻⁴mol/L时，抑制其增殖（分别为55000±1400和59100±1500），（P<0.01）。其他浓度组未见明显影响。
雄性大鼠阴茎海绵体组织中存在AR。睾酮通过AR调节海绵体组织细胞的增殖，不同浓度的睾酮可能与SMCs和成纤维细胞的增殖呈正相关或负相关。
