Chang Chao-Sung, Lin Kuan-Tsao, Chang Jan-Gowth, Lin Chin-Wein, Hsieh Li-Ling, Yeh Chi-Jung, Liu Ta-Chih
Division of Hemato-Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, No. 100, Shih-Chuan 1st Rd, Kaohsiung, Taiwan, ROC.
Kaohsiung Blood Center, Taiwan Blood Services Foundation, Kaohsiung, Taiwan, ROC.
Int J Hematol. 2008 Sep;88(2):127-133. doi: 10.1007/s12185-008-0136-x. Epub 2008 Jul 24.
Molecular genotyping of the ABO alleles has been widely used in ABO subgroups analysis and has been able to solve the rare ABO blood grouping discrepancies. The genotypes of sixty-one A2B phenotype donors recruited from the middle and south of Taiwan were analyzed by means of molecular methods. The A2B phenotype was initially identified by serological test. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to screen the ABO alleles at nucleotides (nt) 261 and 703 based on the nt differences found in the ABO alleles. The subgroups of the A2 allele were determined by the PCR-RFLP and direct sequencing methods. The discrepancies between the phenotype and genotype of the A2B were then studied by subcloning and nucleotide sequence analysis. Our results show that 55 of the 61 A2B donors (90%) are A205/B allele and two are A201/B allele. Four cases were heterozygotes of the cis-AB/O or B alleles. Two were cis-AB04/O allele, one was cis-AB01/O allele and the other was cis-AB02/B allele. In conclusion, most A2B genotypes belong to the A205/B allele in Taiwan. In this study, we report for the first time the presence of the A205, A201, and cis-AB02 alleles in Taiwan.
ABO等位基因的分子基因分型已广泛应用于ABO亚群分析，并能够解决罕见的ABO血型鉴定差异问题。采用分子方法对从台湾中南部招募的61例A2B血型供者的基因型进行了分析。A2B血型最初通过血清学检测确定。基于ABO等位基因中发现的核苷酸差异，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法筛选核苷酸（nt）261和703处的ABO等位基因。通过PCR-RFLP和直接测序方法确定A2等位基因的亚群。然后通过亚克隆和核苷酸序列分析研究A2B血型表型与基因型之间的差异。我们的结果显示，61例A2B供者中有55例（90%）为A205/B等位基因，2例为A201/B等位基因。4例为顺式AB/O或B等位基因的杂合子。2例为顺式AB04/O等位基因，1例为顺式AB01/O等位基因，另1例为顺式AB02/B等位基因。总之，台湾地区大多数A2B基因型属于A205/B等位基因。在本研究中，我们首次报道了台湾地区存在A205、A201和顺式AB02等位基因。
