Almendral-Parra M J, Alonso-Mateos A, Fuentes-Prieto M S
Departamento de Química Analítica, Nutrición y Bromatología, Faculty of Chemistry, University of Salamanca, Plaza de la Merced s/n, 37008, Salamanca, Spain.
J Fluoresc. 2008 Nov;18(6):1169-79. doi: 10.1007/s10895-008-0369-3. Epub 2008 Jul 24.
The use of ozonation for the purification of drinking water can lead to the formation of bromate. The US Environmental Protection Agency and the European Directive for human drinking water has lowered the regulatory level for bromate down to 10 microg l(-1), such that methods must be developed for monitoring the formation of bromate, particularly in on-site situations. In the present work we report a fluorometric method for the determination of bromate based on the reaction with carbostyril-124, a compound that shows fluorescence mainly at pH values above 4 and, when bromated, generates a non-fluorescent product. The reaction can thus be used as an indirect method for determination of the ion. The proposed method, which uses the flow injection (FI) technique, allows online application and kinetic control of the variables affecting the process, together with shorter reaction times, and it provides maximum sensitivity and selectivity. Under optimum conditions, it is possible to determine the analyte within the 4-200 microg l(-1) range, with a limit of detection of 0.9 microg l(-1) and a relative standard deviation (n=12, [BrO3-]=5 and 30 microg l(-1)) of 3.2% and 2.6% respectively. The determination rate was ten samples per hour.
使用臭氧处理净化饮用水会导致溴酸盐的形成。美国环境保护局和欧洲人类饮用水指令已将溴酸盐的监管水平降至10微克/升，因此必须开发监测溴酸盐形成的方法，特别是在现场情况下。在本研究中，我们报告了一种基于与卡唑西林-124反应的荧光法测定溴酸盐，卡唑西林-124是一种主要在pH值高于4时显示荧光的化合物，当被溴化时会生成非荧光产物。因此，该反应可作为测定该离子的间接方法。所提出的方法采用流动注射（FI）技术，允许在线应用和对影响该过程的变量进行动力学控制，同时反应时间更短，并提供最大的灵敏度和选择性。在最佳条件下，能够在4-200微克/升范围内测定分析物，检测限为0.9微克/升，相对标准偏差（n=12，[BrO3-]=5和30微克/升）分别为3.2%和2.6%。测定速率为每小时10个样品。
