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三种体外方法评价葵花籽(Helianthus annuus L.)提取物的抗氧化能力。

Antioxidant capacity of the striped sunflower (Helianthus annuus L.) seed extracts evaluated by three in vitro methods.

机构信息

Department of Basic and Experimental Nutrition, Institute of Nutrition, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

出版信息

Int J Food Sci Nutr. 2009 Aug;60(5):395-401. doi: 10.1080/09637480701755262.

DOI:10.1080/09637480701755262
PMID:18651295
Abstract

The antioxidant capacity of the striped sunflower seed cotyledon extracts, obtained by sequential extraction with different polarities of solvents, was evaluated by three different in vitro methods: ferric reducing/antioxidant power (FRAP), 2.2-diphenyl-1-picrylhydrazyl radical (DPPH) and oxygen radical absorbance capacity (ORAC) assays. In the three methods, the aqueous extract at 30 µg/ml showed a higher antioxidant capacity value (FRAP, 45.27 µmol; DPPH, 50.18%; ORAC, 1.5 Trolox equivalents) than the ethanolic extract (FRAP, 32.17 µmol; DPPH, 15.21%;ORAC, 0.50 Trolox equivalents). When compared with the synthetic antioxidant butylated hydroxyl toluene, the antioxidant capacity of the aqueous extract varied from 45% to 66%, according to the used method. The high antioxidant capacity observed for the aqueous extract of the studied sunflower seed suggests that the intake of this seed may prevent in vivo oxidative reactions responsible for the development of several diseases, such as cancer.

摘要

采用三种不同的体外方法评估了用不同极性溶剂顺序提取的葵花籽胚乳提取物的抗氧化能力

铁还原/抗氧化能力(FRAP)、2,2-二苯基-1-苦基肼基自由基(DPPH)和氧自由基吸收能力(ORAC)测定法。在这三种方法中,浓度为 30μg/ml 的水提物显示出比醇提物更高的抗氧化能力值(FRAP,45.27μmol;DPPH,50.18%;ORAC,1.5 Trolox 当量)(FRAP,32.17μmol;DPPH,15.21%;ORAC,0.50 Trolox 当量)。与合成抗氧化剂叔丁基对羟基甲苯相比,水提物的抗氧化能力根据所使用的方法在 45%到 66%之间变化。研究葵花籽的水提物表现出的高抗氧化能力表明,摄入这种葵花籽可能预防体内负责多种疾病(如癌症)发展的氧化反应。

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