Fan Jinhua, Xie Yingping, Xue Jiaoliang, Li Baozhen
School of Life Science and Technology, Shanxi University, Taiyuan 030006, China.
Wei Sheng Wu Xue Bao. 2008 May;48(5):596-601.
We used submerged fermentation to cultivate a strain of the entomopathogenic fungus Beauveria tenella isolated from the infected larvae of Dentrolimus tabulaeformis in Pinus tabulaeformis forest in Chengde of Hebei Province in China.
We used ethyl acetate to extract antagonistic components from the fermentation broth and used silica gel column chromatography and GC/MS to separate and identify the components.
Six compounds were obtained by silica gel column chromatography. The sixth compound had higher activity to kill the larvae of Dentrolimus tabulaeformis with a corrected mortality rate of 80%. Seventeen compounds were separated and identified by GC/MS in the 6th group, of which 3compounds were more than 10%, 2-Piperidinone (14.02%), 2-coumaranone (47.10%), and Pyrrolo[1,2-a]Pyrazine-1,4-dione, hexahydro (21.05%).
2-Piperidinone and 2-coumaranone had insecticidal activity (corrected mortality rate reached 83.32% and 91.61% respectively) and were the most important toxic substances to control pests.
采用液体深层发酵法培养从河北省承德市油松林中感染油松毛虫幼虫分离得到的一株昆虫病原真菌细脚棒束孢。
用乙酸乙酯从发酵液中提取拮抗成分，采用硅胶柱色谱法和气相色谱 - 质谱联用（GC/MS）法对成分进行分离和鉴定。
通过硅胶柱色谱法得到6种化合物。第6种化合物对油松毛虫幼虫具有较高的杀虫活性，校正死亡率为80%。在第6组中通过GC/MS分离鉴定出17种化合物，其中3种化合物含量超过10%，分别为2 - 哌啶酮（14.02%）、2 - 香豆满酮（47.10%）和六氢吡咯并[1,2 - a]吡嗪 - 1,4 - 二酮（21.05%）。
2 - 哌啶酮和2 - 香豆满酮具有杀虫活性（校正死亡率分别达到83.32%和91.61%），是防治害虫的最重要有毒物质。
