Wiersinga W Joost, Dessing Mark C, van der Poll Tom
Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, The Netherlands.
Microbes Infect. 2008 Jul;10(8):868-77. doi: 10.1016/j.micinf.2008.04.019. Epub 2008 May 7.
Melioidosis, caused by the bacterium Burkholderia pseudomallei, is a septicemic illness, often associated with pneumonia and bacterial dissemination to distant sites. Recently we reported the inflammatory mRNA profile in blood leukocytes during human melioidosis. Knowledge of the inflammatory gene expression profile in the pulmonary compartment after infection with B. pseudomallei, however, is highly limited. We therefore aimed to characterize the inflammatory mRNA profile in the pulmonary and systemic compartment during murine melioidosis. By using a newly developed mouse specific Multiplex-Ligation-dependent-Probe-Amplification (MLPA) assay we determined the expression profile of 33 genes encoding inflammatory proteins in lung tissue, leukocytes in bronchoalveolar-lavage-fluid (BALF) and blood leukocytes in mice before and at several time points after intranasal infection with B. pseudomallei. Relative to naïve mice, mice intranasally infected with B. pseudomallei showed increased transcription of a whole array of genes involved in inflammation, Toll-like receptor-signaling, coagulation, fibrinolysis, cell adhesion, tissue repair and homeostasis in the lung, BALF and blood compartment. Notably, many inflammatory genes were shown to be differentially expressed during the course of infection. These data provide new information on compartmentalized inflammatory gene-expression profiles after infection with B. pseudomallei, increasing our insights into the extent of inflammation activation in the pulmonary and systemic compartment during melioidosis.
类鼻疽是由类鼻疽伯克霍尔德菌引起的一种败血病，常伴有肺炎及细菌播散至远处部位。最近我们报道了人类类鼻疽期间血液白细胞中的炎性mRNA谱。然而，关于感染类鼻疽伯克霍尔德菌后肺组织中炎性基因表达谱的了解非常有限。因此，我们旨在描述小鼠类鼻疽期间肺组织和全身组织中的炎性mRNA谱。通过使用新开发的小鼠特异性多重连接依赖探针扩增（MLPA）检测方法，我们测定了鼻内感染类鼻疽伯克霍尔德菌之前及之后几个时间点小鼠肺组织、支气管肺泡灌洗液（BALF）中的白细胞以及血液白细胞中33个编码炎性蛋白的基因的表达谱。与未感染小鼠相比，鼻内感染类鼻疽伯克霍尔德菌的小鼠在肺组织、BALF和血液组织中，一系列参与炎症、Toll样受体信号传导、凝血、纤维蛋白溶解、细胞黏附、组织修复和内环境稳定的基因转录增加。值得注意的是，许多炎性基因在感染过程中显示出差异表达。这些数据提供了关于感染类鼻疽伯克霍尔德菌后分区炎性基因表达谱的新信息，加深了我们对类鼻疽期间肺组织和全身组织中炎症激活程度的了解。
