Savariar Elamprakash N, Krishnamoorthy K, Thayumanavan S
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Nat Nanotechnol. 2008 Feb;3(2):112-7. doi: 10.1038/nnano.2008.6. Epub 2008 Feb 3.
Recognition of small organic molecules and large biomolecules such as proteins is of great importance in pharmaceutical as well as biological applications. Recognition inside a nanoporous membrane is particularly attractive, because of the advantages associated with ligand-receptor interactions in confined spaces. Classical nanoporous membrane-based separations simply use the difference in size of the analytes relative to pore size in the membrane. In order to bring about selectivity beyond size, it is necessary that methods for functionalizing the membrane pores are readily available. Here, we describe a simple approach to functionalize the nanopores within these membranes using self-assembling and non-self-assembling polymers. We show that these modified membranes separate small molecules based on size, charge and hydrophobicity. We also demonstrate here that proteins can be differentially transported through the nanopores based on their size and/or electrostatics.
识别小分子有机化合物以及蛋白质等大型生物分子在制药和生物学应用中都极为重要。在纳米多孔膜内部进行识别尤其具有吸引力，这是因为在受限空间内配体 - 受体相互作用具有诸多优势。传统的基于纳米多孔膜的分离方法仅仅利用分析物相对于膜孔径大小的差异。为了实现超越尺寸的选择性，必须要有使膜孔功能化的方法。在此，我们描述了一种使用自组装和非自组装聚合物使这些膜内的纳米孔功能化的简单方法。我们表明，这些改性膜基于尺寸、电荷和疏水性来分离小分子。我们还在此证明，蛋白质可以根据其大小和/或静电特性通过纳米孔进行差异传输。
