迈向肉毒杆菌神经毒素A强效抑制剂的发现：开发一种基于液相色谱-质谱联用的稳健检测方法，可在低至亚纳摩尔酶浓度下运行。
Toward the discovery of potent inhibitors of botulinum neurotoxin A: development of a robust LC MS based assay operational from low to subnanomolar enzyme concentrations.
作者信息
Capková Katerina, Hixon Mark S, McAllister Laura A, Janda Kim D
机构信息
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
Chem Commun (Camb). 2008 Aug 14(30):3525-7. doi: 10.1039/b808305c. Epub 2008 Jul 3.
DOI:10.1039/b808305c
PMID:18654701
Abstract
The development of a sensitive, yet reliable assay for the analysis of botulinum neurotoxin A (BoNT/A) inhibitors is described; using this assay a new protease inhibitor was characterized and found to be one of the most potent inhibitors reported to date.
摘要
本文描述了一种用于分析肉毒杆菌神经毒素A（BoNT/A）抑制剂的灵敏且可靠的检测方法；利用该检测方法对一种新型蛋白酶抑制剂进行了表征，发现它是迄今为止报道的最有效的抑制剂之一。
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