Miyamoto Kentaro, Sakurai Hidetaka, Sugiura Takeyuki
Discovery Research Laboratory, Tokyo R&D Center, Daiichi Pharmaceutical Co., Ltd., Daiichi-Sankyo group, Tokyo, Japan.
Proteomics. 2008 Jul;8(14):2907-10. doi: 10.1002/pmic.200800083.
RNF43 is an oncogenic RING finger protein overexpressed in colorectal cancer. To dissect its biological functions, we explored RNF43-interacting proteins by pull-down assay and MS. We identified a heterodimer, p54nrb and PSF, as RNF43's binding partners and confirmed their physical interaction in vivo by the co-immunoprecipitation experiment. Immunofluorescence analysis revealed that co-expression of PSF relocates RNF43 from the nuclear periphery to the nucleoplasm. Thus, proteomic identification of RNF43-associated proteins sheds light on its dynamic interaction network in nuclear events.
RNF43是一种在结直肠癌中过表达的致癌性环状结构域蛋白。为了剖析其生物学功能，我们通过下拉试验和质谱分析探索了与RNF43相互作用的蛋白。我们鉴定出一种异二聚体p54nrb和PSF作为RNF43的结合伴侣，并通过免疫共沉淀实验在体内证实了它们的物理相互作用。免疫荧光分析显示，PSF的共表达使RNF43从核周边重新定位到核质中。因此，对RNF43相关蛋白的蛋白质组学鉴定揭示了其在核事件中的动态相互作用网络。
