基因电转染与佐剂细胞因子在增强丙型肝炎病毒基因疫苗效力方面的协同作用。
Synergistic effect of gene-electro transfer and adjuvant cytokines in increasing the potency of hepatitis C virus genetic vaccination.
作者信息
Arcuri Mirko, Cappelletti Manuela, Zampaglione Immacolata, Aurisicchio Luigi, Nicosia Alfredo, Ciliberto Gennaro, Fattori Elena
机构信息
Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Pomezia, Rome, Italy.
出版信息
J Gene Med. 2008 Sep;10(9):1048-54. doi: 10.1002/jgm.1217.
BACKGROUND
Gene electro-transfer (GET) increases DNA uptake and expression by muscle cells following intramuscular plasmid injection. This technology has been used to increase the production of therapeutic proteins, such as cytokines and growth factors, and to improve immunization efficiency following the injection of antigen-encoding plasmids.
METHODS
Hepatitis C virus (HCV) E2 and cytokine encoding plasmids were co-injected in the mouse quadriceps with or without GET and vaccination outcome was monitored by analysis of antigen-specific cellular-mediated or antibody-mediated immunity.
RESULTS
GET co-injection of cytokine-encoding and HCV E2-encoding plasmids strongly enhanced T- or B-cell responses to various levels, depending on the particular combination used.
CONCLUSIONS
We propose that a cocktail of plasmids followed by GET can be the most efficient and fine-tunable approach for genetic immunization.
背景
基因电穿孔转移（GET）可增加肌肉注射质粒后肌肉细胞对DNA的摄取和表达。该技术已被用于提高治疗性蛋白质（如细胞因子和生长因子）的产量，并在注射编码抗原的质粒后提高免疫效率。
方法
将丙型肝炎病毒（HCV）E2和编码细胞因子的质粒在有或无GET的情况下共注射到小鼠股四头肌中，并通过分析抗原特异性细胞介导或抗体介导的免疫来监测疫苗接种结果。
结果
根据所使用的特定组合，GET共注射编码细胞因子和HCV E2的质粒可在不同水平上强烈增强T细胞或B细胞反应。
结论
我们提出，在GET之后使用质粒混合物可能是基因免疫最有效且可精细调节的方法。
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