[苏云金芽孢杆菌毒素在三种矿物上的吸附与解吸]
[Adsorption and desorption of Bt toxin on three kinds of minerals].
作者信息
Zhou Xue-Yong, Gao Jian-Bao, Cai Peng, Huang Qiao-Yun
机构信息
Key Laboratory of Refractories and Ceramics of Hubei Province, Wuhan University of Science and Technology, Wuhan 430081, China.
出版信息
Ying Yong Sheng Tai Xue Bao. 2008 May;19(5):1144-8.
This paper studied the characteristics of the adsorption and desorption of Bt (Bacillus thuringiensis) toxin on goethite, kaolinite, and silica. The results showed that in phosphate buffer (pH 8), the adsorption isotherms of Bt toxin on the test minerals followed Langmuir equation (R2 >0. 9661), and the adsorbed amounts were in the order of goethite > kaolinite > silica. The Bt toxin was easily adsorbed on the minerals, and the adsorption could reach equilibrium after 1 hour. Within the range of pH 6-8, the amounts of Bt toxin adsorbed on goethite, kaolinite and silica decreased with increasing pH; in the range of 10 degrees C-50 degrees C, the amounts of the toxin adsorbed on goethite and silica decreased by 8.39% and 47.06%, respectively, while that on kaolinite increased slightly (5.91%). The infrared absorption spectrum showed that there was only a minor alteration of Bt toxin after adsorption. The toxin adsorbed on the minerals was not easily desorbed by deionised water, with the desorption rate ranged from 28.48% to 42.04% after three times washing.
本文研究了苏云金芽孢杆菌（Bt）毒素在针铁矿、高岭石和二氧化硅上的吸附和解吸特性。结果表明，在磷酸盐缓冲液（pH 8）中，Bt毒素在受试矿物上的吸附等温线符合朗缪尔方程（R2>0.9661），吸附量顺序为针铁矿>高岭石>二氧化硅。Bt毒素易于吸附在矿物上，1小时后吸附可达到平衡。在pH 6 - 8范围内，针铁矿、高岭石和二氧化硅上吸附的Bt毒素量随pH升高而降低；在10℃ - 50℃范围内，针铁矿和二氧化硅上吸附的毒素量分别降低了8.39%和47.06%，而高岭石上的吸附量略有增加（5.91%）。红外吸收光谱表明，吸附后Bt毒素仅有微小变化。吸附在矿物上的毒素不易被去离子水脱附，三次洗涤后的脱附率为28.48%至42.04%。
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