Wang Chun-You, Gou Shan-Miao, Liu Tao, Wu He-Shui, Xiong Jiong-Xin, Zhou Feng, Tao Jing
Pancreatic Center, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan City, Hubei Province, 430022 China.
Dev Growth Differ. 2008 Oct;50(8):633-43. doi: 10.1111/j.1440-169X.2008.01061.x. Epub 2008 Jul 21.
Pancreatic progenitor cells represent both a potential source of transplantable islets for the treatment of diabetes and a valuable instrument for the investigation of the tumorigenesis of pancreatic carcinoma. It has been reported that pancreatic ductal cells of adults have the characteristics of pancreatic progenitors, but whether these cells can generate endocrine cells requires verification. Here, the differentiation of daughter cells of CD24(-) pancreatic ductal cells into insulin-secreting cells in vitro is reported. Crude pancreatic ductal cells were first obtained from adult mice by gradient centrifugation, and then the CD24(-) cells were isolated with a fluorescence-activated cell sorter. The isolated cells were cultured in serum-containing medium at clonal density to form epithelial colonies (ECs). The ECs were then stimulated with basic fibroblast growth factor (bFGF). After 72 h, insulin-secreting cells were observed in the ECs. These results indicate that the daughter cells of CD24(-) pancreatic ductal cells can differentiate into insulin-secreting cells in vitro when stimulated with exogenous bFGF. Therefore, CD24(-) pancreatic ductal cells have the potential to be pancreatic progenitor cells.
胰腺祖细胞既是用于治疗糖尿病的可移植胰岛的潜在来源，也是研究胰腺癌肿瘤发生的重要工具。据报道，成年个体的胰腺导管细胞具有胰腺祖细胞的特征，但这些细胞是否能产生内分泌细胞仍需验证。在此，报道了体外CD24(-)胰腺导管细胞的子代细胞向胰岛素分泌细胞的分化。首先通过梯度离心从成年小鼠中获得粗制胰腺导管细胞，然后用荧光激活细胞分选仪分离CD24(-)细胞。将分离出的细胞以克隆密度接种于含血清培养基中，形成上皮克隆（ECs）。然后用碱性成纤维细胞生长因子（bFGF）刺激ECs。72小时后，在ECs中观察到胰岛素分泌细胞。这些结果表明，在外源bFGF刺激下，CD24(-)胰腺导管细胞的子代细胞可在体外分化为胰岛素分泌细胞。因此，CD24(-)胰腺导管细胞有潜力成为胰腺祖细胞。
