Valcheva Violeta, Mokrousov Igor, Narvskaya Olga, Rastogi Nalin, Markova Nadya
Department of Pathogenic Bacteria, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
Infect Genet Evol. 2008 Sep;8(5):657-63. doi: 10.1016/j.meegid.2008.06.006. Epub 2008 Jul 9.
We report results of the first study on the molecular basis of drug resistance in Mycobacterium tuberculosis strains currently circulating in Bulgaria. The study panel consisted of 133 (including 37 drug-resistant) isolates recovered from newly diagnosed, adult pulmonary TB patients from different regions of Bulgaria in 2005--2006. Three types of the rpoB mutations were found in 20 of 27 RIF-resistant isolates; rpoB S531L was the most frequent. Eleven (48%) of 23 INH-resistant isolates had katG S315T mutation. inhA -15C > T mutation was detected in one INH-resistant isolate (that also had katG315 mutation) and three INH-susceptible isolates. A mutation in embB306 was found in 7 of 11 EMB-resistant isolates. Comparison with spoligotyping and 24-VNTR locus typing data suggested that emergence and spread of drug-resistant and MDR-TB in Bulgaria are not associated with any specific spoligotype or MIRU-VNTR genotype.
我们报告了关于保加利亚目前流行的结核分枝杆菌菌株耐药性分子基础的首项研究结果。研究小组由2005年至2006年从保加利亚不同地区新诊断的成年肺结核患者中分离出的133株菌株（包括37株耐药菌株）组成。在27株利福平耐药菌株中的20株中发现了三种类型的rpoB突变；rpoB S531L最为常见。23株异烟肼耐药菌株中有11株（48%）发生了katG S315T突变。在1株异烟肼耐药菌株（该菌株也有katG315突变）和3株异烟肼敏感菌株中检测到inhA -15C>T突变。在11株乙胺丁醇耐药菌株中的7株中发现了embB306突变。与 spoligotyping和24-VNTR位点分型数据的比较表明，保加利亚耐药和耐多药结核病的出现和传播与任何特定的spoligotype或MIRU-VNTR基因型无关。
