Terryn Christine, Bonnomet Arnaud, Cutrona Jérôme, Coraux Christelle, Tournier Jean-Marie, Nawrocki-Raby Béatrice, Polette Myriam, Birembaut Philippe, Zahm Jean-Marie
IFR53, Reims, France.
Crit Rev Oncol Hematol. 2009 Feb;69(2):144-52. doi: 10.1016/j.critrevonc.2008.06.005. Epub 2008 Jul 26.
Live-cell imaging has become a powerful analytical tool in most cell biology laboratories. The scope of this paper is to give an overview of the environmental considerations for maintaining living cells on the microscope stage and the technical advances permitting multi-parameter imaging. The paper will then focus on two-dimensional and three-dimensional analysis of cell dispersion and migration and finally give a brief insight on computational modeling of the cell behavior.
活细胞成像已成为大多数细胞生物学实验室中一种强大的分析工具。本文的目的是概述在显微镜载物台上维持活细胞时的环境考量因素以及实现多参数成像的技术进展。然后本文将聚焦于细胞分散和迁移的二维及三维分析，最后简要介绍细胞行为的计算建模。
