Sánchez Samuel, Roldán Mònica, Pérez Sandra, Fàbregas Esteve
Sensors and Biosensors Group, Department of Chemistry, Autonomous University of Barcelona, 08193 Bellaterra, Spain.
Anal Chem. 2008 Sep 1;80(17):6508-14. doi: 10.1021/ac7025282. Epub 2008 Jul 29.
The aim of this study was the fabrication and characterization of biomembranes by the phase inversion (PI) method followed by their subsequent casting onto screen-printed electrodes (SPE) for biomedical applications. The combination of multiwalled carbon nanotubes (MWCNT) as a transducer with polysulfone (PSf) polymer enables easy incorporation of biological moieties (hormones or antibodies), providing a 3D composite with high electrochemical response to corresponding analytes. Antibody/MWCNT/PSf biosensors were characterized by confocal scanning laser microscopy (CSLM), scanning electron microscopy (SEM), and electrochemical methods. For biomedical purposes, human chorionic gonadotropin (hCG) hormone was tested by competitive immunoassay. The detection limit was determined to be 14.6 mIU/mL with a linear range up to 600 mIU/mL. We concluded that the easy and fast incorporation of biomolecules by the PI method, as well as their stability and distribution throughout the 3D polysulfone composite, are testament to the utility for the versatile fabrication of biosensors for clinical diagnosis.
本研究的目的是通过相转化（PI）法制备生物膜并对其进行表征，随后将其浇铸到丝网印刷电极（SPE）上用于生物医学应用。多壁碳纳米管（MWCNT）作为换能器与聚砜（PSf）聚合物相结合，能够轻松引入生物分子（激素或抗体），提供对相应分析物具有高电化学响应的三维复合材料。通过共聚焦扫描激光显微镜（CSLM）、扫描电子显微镜（SEM）和电化学方法对抗体/MWCNT/PSf生物传感器进行了表征。出于生物医学目的，通过竞争性免疫测定法对人绒毛膜促性腺激素（hCG）进行了检测。检测限确定为14.6 mIU/mL，线性范围高达600 mIU/mL。我们得出结论，通过PI法轻松快速地引入生物分子，以及它们在三维聚砜复合材料中的稳定性和分布，证明了其在临床诊断生物传感器通用制造中的实用性。
