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碳纳米管/聚砜复合丝网印刷电化学酶生物传感器

Carbon nanotube/polysulfone composite screen-printed electrochemical enzyme biosensors.

作者信息

Sánchez Samuel, Pumera Martin, Cabruja Enric, Fàbregas Esteve

机构信息

Grup de Sensors i Biosensors, Universitat Autònoma de Barcelona, 08113 Bellaterra, Barcelona, Spain.

出版信息

Analyst. 2007 Feb;132(2):142-7. doi: 10.1039/b609137g. Epub 2006 Nov 9.

Abstract

The fabrication, evaluation and attractive performance of multiwall carbon nanotube(MWCNT)/polysulfone biocomposite membrane modified thick-film screen-printed electrochemical biosensors are reported. The fabricated carbon nanotube/polysulfone (CNT/PS) strips combine the attractive advantages of carbon nanotube materials, polysulfone matrix and disposable screen-printed electrodes. Such thick-film carbon nanotubes/polysulfone sensors have a well defined performance, are mechanically stable, and exhibit high electrochemical activity. Furthermore, biocompatibility of CNT/PS composite allows easy incorporation of biological functional moiety of horseradish peroxidase by phase inversion technique. The comparison of graphite with MWCNT as conductor material is described in this paper. The proposed H(2)O(2) biosensor exhibited a linear range (applied potential, -0.2 V) from 0.02 to 0.5 mM and a K(M)(app) of 0.71 mM.

摘要

报道了多壁碳纳米管(MWCNT)/聚砜生物复合膜修饰厚膜丝网印刷电化学生物传感器的制备、评估及优异性能。所制备的碳纳米管/聚砜(CNT/PS)条带结合了碳纳米管材料、聚砜基质和一次性丝网印刷电极的诱人优势。此类厚膜碳纳米管/聚砜传感器具有明确的性能,机械稳定性良好,并展现出高电化学活性。此外,CNT/PS复合材料的生物相容性使得通过相转化技术能够轻松掺入辣根过氧化物酶的生物功能部分。本文描述了将石墨与MWCNT作为导体材料的比较。所提出的H₂O₂生物传感器在应用电位为 -0.2 V时,线性范围为0.02至0.5 mM,表观米氏常数(Kₘₐₚ)为0.71 mM。

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