Simchuk A P
Genetika. 2008 Apr;44(4):488-95.
The effect of genetic variation of oak (Quercus pubescens L. and Q. petraea L.) on the genotype fitness components in green oak leafroller moth larvae (Tortrix viridana L.) at esterase (Est-4) and protease (Pts-4) loci was studied. The samples of larvae were collected from nine oak trees, whose genetic variation was assayed by RAPD-PCR using primer OPA14. The contributions of the factors of Yoak species/genotype and green oak leafroller moth genotype and their interaction to the variation of important size-related traits of the larvae were evaluated by two-way ANOVA. It was shown that the same larval genotype can display maximum fitness on the trees of one species or genotype and minimal, on the trees of other species or genotype. The interactions between the oak genotype and green oak leafroller moth genotype factors lead to the relationships that appear in statistically significant associations between genotype classes of green oak leafroller moth and oak. These results are discussed from the standpoint of a recently developed new field, community or ecosystem genetics.
研究了栎树（柔毛栎和无梗花栎）的遗传变异对绿卷叶蛾幼虫在酯酶（Est-4）和蛋白酶（Pts-4）位点上的基因型适合度组分的影响。从9棵栎树上采集幼虫样本，使用引物OPA14通过RAPD-PCR分析其遗传变异。通过双向方差分析评估栎树种类/基因型和绿卷叶蛾基因型因素及其相互作用对幼虫重要的大小相关性状变异的贡献。结果表明，相同的幼虫基因型在一种栎树种类或基因型的树上可表现出最大适合度，而在其他种类或基因型的树上则表现出最小适合度。栎树基因型和绿卷叶蛾基因型因素之间的相互作用导致了绿卷叶蛾基因型类与栎树之间在统计上显著关联的关系。从最近发展起来的新领域——群落或生态系统遗传学的角度对这些结果进行了讨论。
