Long H, Huang Z, Wei Y-M, Yan Z-H, Ma Z-C, Zheng Y-L
Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan, China.
Genetika. 2008 Apr;44(4):500-6.
Allelic variation of the low-molecular-weight glutenin subunit (LMW-GS) is associated with the significant differences of dough quality in bread and durum wheat, and has been widely evaluated at protein level in wheat and its relatives. In this study, a PCR primer set, targeting the high variable repetitive domains, was employed to assay the length variation of i-type LMW-GS genes in the A-genomes of diploid wheats, the diploid progenitors of tetraploid and hexaploid wheat. A total of 71 accessions of diploid wheats, belonging to two wild and one cultivated species, were investigated. The higher variations of repetitive length in i-type LMW-GS genes were found in diploid wheats with Nei's genetic variation index (H) of 0.834. The two wild species, T. boeoticum and T. urartu, were found to possess the similar degree of variability, with the Nei's genetic variation index of 0.806 and 0.783, respectively. Less variations were detected in T. monococcum (H = 0.680), a cultivated species domesticated from T. boeoticum. The sufficient variations found in this study could be used as valuable sources for the enrichment of the genetic variations and the alteration of flour-processing properties of the cultivated wheat. To our knowledge, it was the first time that an analysis of length variation targeting a particular group of genes of LMW-GS complex multigene families was conducted.
低分子量谷蛋白亚基（LMW-GS）的等位变异与面包小麦和硬粒小麦面团品质的显著差异相关，并且已在小麦及其近缘种的蛋白质水平上得到广泛评估。在本研究中，使用一组针对高变重复结构域的PCR引物，来检测二倍体小麦A基因组中i型LMW-GS基因的长度变异，二倍体小麦是四倍体和六倍体小麦的二倍体祖先。共研究了属于两个野生种和一个栽培种的71份二倍体小麦材料。在二倍体小麦中发现i型LMW-GS基因的重复长度变异较高，内氏遗传变异指数（H）为0.834。发现两个野生种，即野生一粒小麦和乌拉尔图小麦，具有相似的变异程度，内氏遗传变异指数分别为0.806和0.783。在由野生一粒小麦驯化而来的栽培种栽培一粒小麦中检测到的变异较少（H = 0.680）。本研究中发现的丰富变异可作为宝贵资源，用于丰富栽培小麦的遗传变异和改变其面粉加工特性。据我们所知，这是首次针对LMW-GS复合多基因家族的特定基因群进行长度变异分析。
