Kamiński S, Malewski T, Ahman A, Wójcik E, Ruść A, Oleński K, Jakubczak A, Sazanov A A
Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn 10-719, Poland.
Genetika. 2008 Apr;44(4):532-8.
MilkProtChip is oligonucleotide microarray allowing bovine genotyping based on single nucleotide polymorphisms (SNPs) in genes influencing milk protein biosynthesis. A total of 71 SNPs in 42 genes were selected as associated with milk protein biosynthesis. Genotyping of about 300 animals of Polish Black-and-White cattle showed that SNPs in acyl-CoA: 1,2-diacylglycerol O-transferase (DGAT1), lactoferrin (LTF), casein kappa (CSN3) and growth hormone receptor (GHR) genes were associated with several milk performance traits. Analysis of correlations between SNPs and milk production traits showed that SNPs in single genes rarely affect the investigated traits. Only 4 of 42 investigated single SNPs had impact on milk production traits while 22 combinations of paired SNPs in these genes had impact. Positive effect SNP combinations in two genes can be a result of additive effect on these SNPs on the same traits or effect of genes interaction. The MilkBovExp chip representing 90 genes encoding transcription factors expressed in the bovine mammary gland and/or involved in mammary gland signaling pathways was designed for further investigation of impact of gene expression and/or its encoded products on milk traits performance.
牛奶蛋白芯片是一种寡核苷酸微阵列，可基于影响牛奶蛋白生物合成的基因中的单核苷酸多态性（SNP）对牛进行基因分型。总共选择了42个基因中的71个SNP，它们与牛奶蛋白生物合成相关。对约300头波兰黑白花奶牛进行基因分型，结果表明，酰基辅酶A：1,2 - 二酰甘油O - 转移酶（DGAT1）、乳铁蛋白（LTF）、酪蛋白κ（CSN3）和生长激素受体（GHR）基因中的SNP与多个产奶性能性状相关。SNP与产奶性状之间的相关性分析表明，单个基因中的SNP很少影响所研究的性状。在42个被研究的单个SNP中，只有4个对产奶性状有影响，而这些基因中22对SNP组合有影响。两个基因中的正向效应SNP组合可能是这些SNP对同一性状的加性效应或基因相互作用的结果。代表90个在牛乳腺中表达和/或参与乳腺信号通路的转录因子编码基因的牛奶牛表达芯片，被设计用于进一步研究基因表达和/或其编码产物对产奶性状表现的影响。
