3个新型猪基因ASPA、NAGA和HEXA的分离、序列鉴定及组织表达谱
Isolation, sequence identification and tissue expression profiles of 3 novel porcine genes: ASPA, NAGA, and HEXA.
作者信息
Shu Xianghua, Liu Yonggang, Yang Liangyu, Song Chunlian, Hou Jiafa
机构信息
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
出版信息
J Appl Genet. 2008;49(3):257-61. doi: 10.1007/BF03195622.
The complete coding sequences of 3 porcine genes - ASPA, NAGA, and HEXA - were amplified by the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved sequence information of the mouse or other mammals and referenced pig ESTs. These 3 novel porcine genes were then deposited in the NCBI database and assigned GeneIDs: 100142661, 100142664 and 100142667. The phylogenetic tree analysis revealed that the porcine ASPA, NAGA, and HEXA all have closer genetic relationships with the ASPA, NAGA, and HEXA of cattle. Tissue expression profile analysis was also carried out and results revealed that swine ASPA, NAGA, and HEXA genes were differentially expressed in various organs, including skeletal muscle, the heart, liver, fat, kidney, lung, and small and large intestines. Our experiment is the first one to establish the foundation for further research on these 3 swine genes.
基于小鼠或其他哺乳动物的保守序列信息以及参考猪的ESTs，通过逆转录聚合酶链反应（RT-PCR）扩增了3个猪基因——ASPA、NAGA和HEXA的完整编码序列。然后将这3个新的猪基因存入NCBI数据库，并被赋予基因ID：100142661、100142664和100142667。系统发育树分析表明，猪的ASPA、NAGA和HEXA与牛的ASPA、NAGA和HEXA都有更近的亲缘关系。还进行了组织表达谱分析，结果显示猪的ASPA、NAGA和HEXA基因在包括骨骼肌、心脏、肝脏、脂肪、肾脏、肺以及小肠和大肠在内的各种器官中存在差异表达。我们的实验首次为进一步研究这3个猪基因奠定了基础。
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