Liu G Y
Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China.
J Appl Genet. 2009;50(1):47-50. doi: 10.1007/BF03195651.
The complete coding sequences of porcine genes NCF2, BCKDHB and BCKDHA were amplified by using reverse transcriptase polymerase chain reaction (RT-PCR), basing on the conserved coding sequence information of humans or other mammals. These 3 novel porcine genes were then assigned GeneIDs: 100142665, 100142669 and 100142666. The phylogenetic tree analysis revealed that the porcine NCF2, BCKDHB and BCKDHA all are most closely related to the bovine NCF2, BCKDHB and BCKDHA. Tissue expression profile analysis revealed that porcine NCF2, BCKDHB and BCKDHA genes were differentially expressed in tissues, including skeletal muscle, the heart, liver, fat, kidney, lung, small and large intestine.
基于人类或其他哺乳动物的保守编码序列信息，采用逆转录聚合酶链反应（RT-PCR）扩增猪基因NCF2、BCKDHB和BCKDHA的完整编码序列。然后为这3个新的猪基因指定了基因ID：100142665、100142669和100142666。系统发育树分析表明，猪的NCF2、BCKDHB和BCKDHA与牛的NCF2、BCKDHB和BCKDHA关系最为密切。组织表达谱分析表明，猪的NCF2、BCKDHB和BCKDHA基因在骨骼肌、心脏、肝脏、脂肪、肾脏、肺、小肠和大肠等组织中存在差异表达。
Zotero 插件